Feeder-free derivation of melanocytes from human pluripotent stem cells Journal Article
| Authors: | Callahan, S. J.; Mica, Y.; Studer, L. |
| Article Title: | Feeder-free derivation of melanocytes from human pluripotent stem cells |
| Abstract: | Human pluripotent stem cells (hPSCs) represent a platform to study human development in vitro under both normal and disease conditions. Researchers can direct the differentiation of hPSCs into the cell type of interest by manipulating the culture conditions to recapitulate signals seen during development. One such cell type is the melanocyte, a pigment-producing cell of neural crest (NC) origin responsible for protecting the skin against UV irradiation. This protocol presents an extension of a currently available in vitro Neural Crest differentiation protocol from hPSCs to further differentiate NC into fully pigmented melanocytes. Melanocyte precursors can be enriched from the Neural Crest protocol via a timed exposure to activators of WNT, BMP, and EDN3 signaling under dual-SMAD-inhibition conditions. The resultant melanocyte precursors are then purified and matured into fully pigmented melanocytes by culture in a selective medium. The resultant melanocytes are fully pigmented and stain appropriately for proteins characteristic of mature melanocytes. © 2016 Journal of Visualized Experiments. |
| Keywords: | melanocytes; developmental biology; pigmentation; neural crest; human embryonic stem cells; disease modeling; in vitro differentiation; melanoblast; embryonic stem cells (escs); induced pluripotent stem cells (ipscs); differentiation protocol; human pluripotent stem cells (hpscs); issue 109 |
| Journal Title: | Journal of Visualized Experiments |
| Volume: | 109 |
| ISSN: | 1940-087X |
| Publisher: | MYJoVE Corporation |
| Date Published: | 2016-03-03 |
| Start Page: | e53806 |
| Language: | English |
| DOI: | 10.3791/53806 |
| PROVIDER: | scopus |
| PUBMED: | 26967464 |
| PMCID: | PMC4828213 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 May 2016 -- Source: Scopus |
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