Cyclic microchip assay for measurement of hundreds of functional proteins in single neurons Journal Article

   

Authors:	Yang, L.; Ball, A.; Liu, J.; Jain, T.; Li, Y. M.; Akhter, F.; Zhu, D.; Wang, J.
Article Title:	Cyclic microchip assay for measurement of hundreds of functional proteins in single neurons
Abstract:	Despite the fact that proteins carry out nearly all cellular functions and mark the differences of cells, the existing single-cell tools can only analyze dozens of proteins, a scale far from full characterization of cells and tissue yet. Herein, we present a single-cell cyclic multiplex in situ tagging (CycMIST) technology that affords the comprehensive functional proteome profiling of single cells. We demonstrate the technology by detecting 182 proteins that include surface markers, neuron function proteins, neurodegeneration markers, signaling pathway proteins, and transcription factors. Further studies on cells derived from the 5XFAD mice, an Alzheimer’s Disease (AD) model, validate the utility of our technology and reveal the deep heterogeneity of brain cells. Through comparison with control mouse cells, we have identified differentially expressed proteins in AD pathology. Our technology could offer new insights into cell machinery and thus may advance many fields including drug discovery, molecular diagnostics, and clinical studies. © 2022, The Author(s).
Keywords:	controlled study; protein expression; nonhuman; biomarkers; biological marker; protein analysis; proteome; animal cell; mouse; animal; metabolism; animals; mice; fluorescent dye; animal experiment; animal model; astrocyte; protein; neurons; immunofluorescence; drug development; assay; transgenic mouse; mice, transgenic; disease model; brain; immunogenicity; disease models, animal; tubulin; cd24 antigen; nerve cell; phosphoprotein; alzheimer disease; machinery; cd86 antigen; amyloid precursor protein; presenilin 1; cyclic amp responsive element binding protein; nerve degeneration; functional proteomics; cell; heterogeneity; prefrontal cortex; oligodendroglia; antibody conjugate; brain cell; cell membrane protein; microtubule associated protein 2; protein content; lamin b; male; article; hydroxymethylglutaryl coenzyme a reductase; triggering receptor expressed on myeloid cells 2; vesicular glutamate transporter 2; 5xfad mouse; microchip analysis; neuro-2a cell line
Journal Title:	Nature Communications
Volume:	13
ISSN:	2041-1723
Publisher:	Nature Publishing Group
Date Published:	2022-06-21
Start Page:	3548
Language:	English
DOI:	10.1038/s41467-022-31336-x
PUBMED:	35729174
PROVIDER:	scopus
PMCID:	PMC9213506
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132278408&doi=10.1038%2fs41467-022-31336-x&partnerID=40&md5=1d2bf336d088e895f7a5caaaf1685da7
Notes:	Article -- Export Date: 1 August 2022 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/196319