GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst Journal Article

   

Authors:	Schrode, N.; Saiz, N.; Di Talia, S.; Hadjantonakis, A. K.
Article Title:	GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst
Abstract:	Cells of the inner cell mass (ICM) of the mouse blastocyst differentiate into the pluripotent epiblast or the primitive endoderm (PrE), marked by the transcription factors NANOG and GATA6, respectively. To investigate the mechanistic regulation of this process, we applied an unbiased, quantitative, single-cell-resolution image analysis pipeline to analyze embryos lacking or exhibiting reduced levels of GATA6. We find that Gata6 mutants exhibit a complete absence of PrE and demonstrate that GATA6 levels regulate the timing and speed of lineage commitment within the ICM. Furthermore, we show that GATA6 is necessary for PrE specification by FGF signaling and propose a model where interactions between NANOG, GATA6, and the FGF/ERK pathway determine ICM cell fate. This study provides a framework for quantitative analyses of mammalian embryos and establishes GATA6 as a nodal point in the gene regulatory network driving ICM lineage specification. © 2014 Elsevier Inc.
Keywords:	immunohistochemistry; signal transduction; mitogen activated protein kinase; nonhuman; mouse; mammalia; gene expression; embryo; genotype; cell fate; cell differentiation; immunofluorescence; cell lineage; quantitative analysis; cell count; upregulation; gene regulatory network; cell interaction; cell communication; fibroblast growth factor; transcription factor nanog; endoderm; blastocyst; transcription factor gata 6; inner cell mass; single cell analysis; transient expression; priority journal; article
Journal Title:	Developmental Cell
Volume:	29
Issue:	4
ISSN:	1534-5807
Publisher:	Cell Press
Date Published:	2014-05-27
Start Page:	454
End Page:	467
Language:	English
DOI:	10.1016/j.devcel.2014.04.011
PROVIDER:	scopus
PUBMED:	24835466
PMCID:	PMC4103658
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84901627728&partnerID=40&md5=0edb339bbff76e6a2ae7a29333aa9aba
Notes:	Dev. Cell -- Export Date: 8 July 2014 -- CODEN: DCEEB -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/54038