Lineage establishment and progression within the inner cell mass of the mouse blastocyst requires FGFR1 and FGFR2 Journal Article


Authors: Kang, M.; Garg, V.; Hadjantonakis, A. K.
Article Title: Lineage establishment and progression within the inner cell mass of the mouse blastocyst requires FGFR1 and FGFR2
Abstract: Fibroblast growth factor 4 (FGF4) is the key signal driving specification of primitive endoderm (PrE) versus pluripotent epiblast (EPI) within the inner cell mass (ICM) of the mouse blastocyst. To gain insight into the receptor(s) responding to FGF4 within ICM cells, we combined single-cell-resolution quantitative imaging with single-cell transcriptomics of wild-type and Fgf receptor (Fgfr) mutant embryos. Despite the PrE-specific expression of FGFR2, it is FGFR1, expressed by all ICM cells, that is critical for establishment of a PrE identity. Signaling through FGFR1 is also required to constrain levels of the pluripotency-associated factor NANOG in EPI cells. However, the activity of both receptors is required for lineage establishment within the ICM. Gene expression profiling of 534 single ICM cells identified distinct downstream targets associated with each receptor. These data lead us to propose a model whereby unique and additive activities of FGFR1 and FGFR2 within the ICM coordinate establishment of two distinct lineages. © 2017 Elsevier Inc.
Keywords: blastocyst; epiblast; primitive endoderm; inner cell mass; quantitative image analysis; fgf; pluripotency; nanog; gata6; single-cell transcriptomics
Journal Title: Developmental Cell
Volume: 41
Issue: 5
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2017-06-05
Start Page: 496
End Page: 510.e5
Language: English
DOI: 10.1016/j.devcel.2017.05.003
PROVIDER: scopus
PUBMED: 28552559
PMCID: PMC5530874
DOI/URL:
Notes: Article -- Export Date: 3 July 2017 -- Source: Scopus
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MSK Authors
  1. Vidur Garg
    6 Garg
  2. Min Jung Kang
    11 Kang