Heterogeneities in nanog expression drive stable commitment to pluripotency in the mouse blastocyst Journal Article

   


Authors: Xenopoulos, P.; Kang, M.; Puliafito, A.; DiTalia, S.; Hadjantonakis, A. K.
Article Title: Heterogeneities in nanog expression drive stable commitment to pluripotency in the mouse blastocyst
Abstract: The pluripotent epiblast (EPI) is the founder tissue of almost all somatic cells. EPI and primitive endoderm (PrE) progenitors arise from the inner cell mass (ICM) of the blastocyst-stage embryo. The EPI lineage isdistinctly identified by its expression of pluripotency-associated factors. Many of these factors have been reported to exhibit dynamic fluctuations of expression in embryonic stem cell cultures. Whether these fluctuations correlating with ICM fate choice occur invivo remains an open question. Using single-cell resolution quantitative imaging of a Nanog transcriptional reporter, we noted an irreversible commitment to EPI/PrE lineages invivo. A period of apoptosis occurred concomitantly with ICM cell-fate choice, followed by a burst of EPI-specific cell proliferation. Transitions were occasionally observed from PrE-to-EPI, but not vice versa, suggesting that they might be regulated and not stochastic. We propose that the rapid timescale of early mammalian embryonic development prevents fluctuations in cell fate. © 2015 The Authors.
Keywords: controlled study; protein expression; nonhuman; protein localization; cell proliferation; animal cell; mouse; mammalia; animal tissue; apoptosis; embryo; embryonic stem cell; molecular dynamics; animal experiment; cell differentiation; immunofluorescence; animal husbandry; cell lineage; quantitative analysis; reporter gene; down regulation; pluripotent stem cell; fluorescence activated cell sorting; transcription factor nanog; genetic heterogeneity; blastocyst; inner cell mass; priority journal; article
Journal Title: Cell Reports
Volume: 10
Issue: 9
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2015-03-10
Start Page: 1508
End Page: 1520
Language: English
DOI: 10.1016/j.celrep.2015.02.010
PROVIDER: scopus
PUBMED: 25753417
PMCID: PMC4560681
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84924532692&partnerID=40&md5=b0c8c6f044aef0184c56195bd684a411
Notes: Export Date: 2 April 2015 -- Source: Scopus
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MSK Authors
  1. Anna-Katerina Hadjantonakis
    191 Hadjantonakis
  2. Panagiotis Xenopoulos
    11 Xenopoulos
  3. Min Jung Kang
    12 Kang
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