The primitive endoderm lineage of the mouse blastocyst: Sequential transcription factor activation and regulation of differentiation by Sox17 Journal Article

Authors: Artus, J.; Piliszek, A.; Hadjantonakis, A. K.
Article Title: The primitive endoderm lineage of the mouse blastocyst: Sequential transcription factor activation and regulation of differentiation by Sox17
Abstract: Cells of the primitive endoderm (PrE) and the pluripotent epiblast (EPI), the two lineages specified within the inner cell mass (ICM) of the mouse blastocyst stage embryo, are segregated into adjacent tissue layers by the end of the preimplantation period. The PrE layer which emerges as a polarized epithelium adjacent to the blastocoel, with a basement membrane separating it from the EPI, has two derivatives, the visceral and parietal endoderm. In this study we have investigated the localization of two transcriptional regulators of the SOX family, SOX17 and SOX7, within the PrE and its derivatives. We noted that SOX17 was first detected in a salt-and-pepper distribution within the ICM, subsequently becoming restricted to the nascent PrE epithelium. This dynamic distribution of SOX17 resembled the localization of GATA6 and GATA4, two other PrE lineage-specific transcription factors. By contrast, SOX7 was only detected in PrE cells positioned in contact with the blastocoel, raising the possibility that these cells are molecularly distinct. Our observations support a model of sequential GATA6 > SOX17 > GATA4 > SOX7 transcription factor activation within the PrE lineage, perhaps correlating with the consecutive periods of cell lineage 'naïvete', commitment and sorting. Furthermore our data suggest that co-expression of SOX17 and SOX7 within sorted PrE cells could account for the absence of a detectable phenotype of Sox17 mutant blastocysts. However, analysis of implantation-delayed blastocysts, revealed a role for SOX17 in the maintenance of PrE epithelial integrity, with the absence of SOX17 leading to premature delamination and migration of parietal endoderm. © 2010.
Keywords: controlled study; nonhuman; animal cell; mouse; animal tissue; embryo; transcription initiation; gene function; cell differentiation; cell lineage; gene expression regulation; transcription regulation; gene identification; cellular distribution; pluripotent; epithelium; gene location; cell selection; endoderm; mouse embryo; blastocyst; genetic correlation; confocal imaging; epiblast; implantation delay; primitive endoderm; sox17; sox7; transcription factor gata 4; transcription factor gata 6; transcription factor sox17; transcription factor sox7; blastocyte; inner cell mass
Journal Title: Developmental Biology
Volume: 350
Issue: 2
ISSN: 0012-1606
Publisher: Elsevier Inc.  
Date Published: 2011-02-15
Start Page: 393
End Page: 404
Language: English
DOI: 10.1016/j.ydbio.2010.12.007
PROVIDER: scopus
PUBMED: 21146513
PMCID: PMC3461954
Notes: --- - "Export Date: 4 March 2011" - "CODEN: DEBIA" - "Source: Scopus"
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  1. Jerome Francois Artus
    14 Artus