Live imaging mouse embryonic development: Seeing is believing and revealing Journal Article


Authors: Nowotschin, S.; Hadjantonakis, A. K.
Article Title: Live imaging mouse embryonic development: Seeing is believing and revealing
Abstract: The use of genetically encoded fluorescent proteins has revolutionized the fields of cell and developmental biology and redefined our understanding of the dynamic morphogenetic processes that work to shape the embryo. Fluorescent proteins are routinely used as vital reporters to label tissues, cells, cellular organelles, or proteins of interest and in doing so provide contrasting agents enabling the acquisition of high-resolution quantitative image data. With the advent of more accessible and sophisticated imaging technologies and abundance of fluorescent proteins with different spectral characteristics, the dynamic processes taking place in situ in living embryos can now be probed. Here, we provide an overview of some recent advances in this rapidly evolving field. © 2014 Springer Science+Business Media, New York.
Keywords: gfp; green fluorescent protein; red fluorescent protein; transgenic; rfp; live imaging; mouse embryo; time-lapse; 3d; reporter; gene targeted; genetically encoded fluorescent protein
Journal Title: Methods in Molecular Biology
Volume: 1092
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2014-01-01
Start Page: 405
End Page: 420
Language: English
DOI: 10.1007/978-1-60327-292-6_24
PROVIDER: scopus
PMCID: PMC3967567
PUBMED: 24318833
DOI/URL:
Notes: Chapter 24 in "Mouse Molecular Embryology: Methods and Protocols" (ISBN: 978-1-60327-290-2) -- Export Date: 3 February 2014 -- Source: Scopus
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