Live imaging of Drosophila myoblast fusion Journal Article
| Authors: | Richardson, B. E.; Beckett, K.; Baylies, M. K. |
| Article Title: | Live imaging of Drosophila myoblast fusion |
| Abstract: | Myoblast fusion requires a number of cellular behaviors, including cell migration, recognition, and adhesion, as well as a series of subcellular behaviors, such as cytoskeletal rearrangements, vesicle trafficking, and membrane dynamics, leading to two cells becoming one. With the discovery of fluorescent proteins that can be introduced and studied within living cells, the possibility of monitoring these complex processes within the living embryo is now a reality. Live imaging, unlike imaging techniques for fixed embryos, allows the opportunity to visualize and measure the dynamics of these processes in vivo. This chapter describes the development and use of live imaging techniques to study myoblast fusion in Drosophila. © 2008 Humana Press. |
| Keywords: | promoter region; methodology; animal; cytology; metabolism; animals; cell survival; confocal microscopy; microscopy, confocal; drosophila; photoprotein; luminescent proteins; animal embryo; prenatal development; embryo, nonmammalian; promoter regions, genetic; drosophila melanogaster; computer program; software; live imaging; myoblast fusion; cell fusion; myoblast; fluorescent proteins; myoblasts; muscle development; enhancer region; enhancer elements, genetic |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 475 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2008-01-01 |
| Start Page: | 263 |
| End Page: | 274 |
| Language: | English |
| DOI: | 10.1007/978-1-59745-250-2_15 |
| PUBMED: | 18979249 |
| PROVIDER: | scopus |
| PMCID: | PMC2883172 |
| DOI/URL: | |
| Notes: | Chapter 15 in "Cell Fusion: Overviews and Methods" (ISBN: 978-1-58829-911-6) - Cited By (since 1996): 4 - Export Date: 17 November 2011 - Source: Scopus |
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