Ex utero culture and live imaging of mouse embryos Journal Article

   


Authors: Piliszek, A.; Kwon, G. S.; Hadjantonakis, A. K.
Article Title: Ex utero culture and live imaging of mouse embryos
Abstract: Mouse genetic approaches when combined with live imaging tools have the potential to revolutionize our current understanding of mammalian biology. The availability and improvement of a wide variety of fluorescent proteins have provided indispensable tools to visualize cells in living organisms. It is now possible to generate genetically modified mouse strains expressing fluorescent proteins in a tissue-specific manner. These reporter-expressing strains make it possible to image dynamic cell behaviors in the context of a living embryo. Since mouse embryos develop within the uterus, live imaging experiments require culture conditions that closely mimic those in vivo. Over the past few decades, significant advances have been made in developing conditions for culturing both pre- and postimplantation stage embryos. In this chapter, we will discuss methods for ex utero culture of preimplantation and postimplantation stage mouse embryos. In particular, we will describe protocols for collecting embryos at various stages, setting up culture conditions for imaging and using laser scanning confocal microscopy to visualize live processes in mouse embryos expressing fluorescent reporters. © 2011 Springer Science+Business Media, LLC.
Keywords: mammalia; live imaging; mouse embryo; fluorescent protein; ex utero culture; time lapse
Journal Title: Methods in Molecular Biology
Volume: 770
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2011-01-01
Start Page: 243
End Page: 257
Language: English
DOI: 10.1007/978-1-61779-210-6_9
PROVIDER: scopus
PUBMED: 21805267
PMCID: PMC3298811
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-80052944694&partnerID=40&md5=adcdd2e68dd2b290d41c577039e9e8b9
Notes: Chapter 9 in "Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods" (ISBN: 978-1-61779-209-0) --- Export Date: 3 October 2011 - Source: Scopus
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MSK Authors
  1. Gloria S Kwon
    9 Kwon
  2. Anna-Katerina Hadjantonakis
    193 Hadjantonakis
  3. Anna Ewa Piliszek
    11 Piliszek
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