Live imaging fluorescent proteins in early mouse embryos Journal Article
| Authors: | Xenopoulos, P.; Nowotschin, S.; Hadjantonakis, A. K. |
| Article Title: | Live imaging fluorescent proteins in early mouse embryos |
| Abstract: | Mouse embryonic development comprises highly dynamic and coordinated events that drive key cell lineage specification and morphogenetic events. These processes involve cellular behaviors including proliferation, migration, apoptosis, and differentiation, each of which is regulated both spatially and temporally. Live imaging of developing embryos provides an essential tool to investigate these coordinated processes in three-dimensional space over time. For this purpose, the development and application of genetically encoded fluorescent protein (FP) reporters has accelerated over the past decade allowing for the high-resolution visualization of developmental progression. Ongoing efforts are aimed at generating improved reporters, where spectrally distinct as well as novel FPs whose optical properties can be photomodulated, are exploited for live imaging of mouse embryos. Moreover, subcellular tags in combination with using FPs allow for the visualization of multiple subcellular characteristics, such as cell position and cell morphology, in living embryos. Here, we review recent advances in the application of FPs for live imaging in the early mouse embryo, as well as some of the methods used for ex utero embryo development that facilitate on-stage time-lapse specimen visualization. © 2012 Elsevier Inc. All rights reserved. |
| Keywords: | microscopy; nonhuman; cell proliferation; protein analysis; mouse; animals; mice; cell function; cell structure; apoptosis; embryo; fluorescence; embryo development; morphogenesis; cell differentiation; cell lineage; cell specificity; luminescent proteins; imaging system; regulatory mechanism; recombinant fusion proteins; cell migration; embryo, mammalian; organ culture techniques; live imaging; mouse embryo; fluorescent protein; enhanced green fluorescent protein; yellow fluorescent protein; cyan fluorescent protein; keywords |
| Journal Title: | Methods in Enzymology |
| Volume: | 506 |
| ISSN: | 0076-6879 |
| Publisher: | Academic Press |
| Date Published: | 2012-01-01 |
| Start Page: | 361 |
| End Page: | 389 |
| Language: | English |
| DOI: | 10.1016/b978-0-12-391856-7.00042-1 |
| PROVIDER: | scopus |
| PUBMED: | 22341233 |
| PMCID: | PMC4955835 |
| DOI/URL: | |
| Notes: | Chapter 18 in "Imaging and Spectroscopic Analysis of Living Cells: Imaging Live Cells in Health and Disease" (ISBN: 978-0-12-391856-7) - "Export Date: 2 April 2012" - "CODEN: MENZA" - "Source: Scopus" |
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191Hadjantonakis -
11Xenopoulos
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