Ex utero culture and imaging of mouse embryos Journal Article

  


Authors: Nowotschin, S.; Garg, V.; Piliszek, A.; Hadjantonakis, A. K.
Article Title: Ex utero culture and imaging of mouse embryos
Abstract: Mouse genetic approaches when combined with live imaging tools are revolutionizing our current understanding of mammalian developmental biology. The availability and improvement of a wide variety of genetically encoded fluorescent proteins have provided indispensable tools to visualize cells and subcellular features in living organisms. It is now possible to generate genetically modified mouse lines expressing several spectrally distinct fluorescent proteins in a tissue-specific or -inducible manner. Such reporter-expressing lines make it possible to image dynamic cellular behaviors in the context of living embryos undergoing normal or aberrant development. As with all viviparous mammals, mouse embryos develop within the uterus, and so live imaging experiments require culture conditions that closely mimic the in vivo environment. Over the past decades, significant advances have been made in developing conditions for culturing both pre- and postimplantation-stage mouse embryos. In this chapter, we discuss routine methods for ex utero culture of preimplantation- and postimplantation-stage mouse embryos. In particular, we describe protocols for collecting mouse embryos of various stages, setting up culture conditions for their ex utero culture and imaging, and using laser scanning confocal microscopy to visualize live processes in mouse embryos expressing fluorescent reporters.
Keywords: confocal; gfp; imaging; rfp; mouse embryo; fluorescent protein; ex utero culture; time-lapse; reporter
Journal Title: Methods in Molecular Biology
Volume: 1920
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2019-01-01
Start Page: 163
End Page: 182
Language: English
DOI: 10.1007/978-1-4939-9009-2_11
PUBMED: 30737692
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061275197&doi=10.1007%2f978-1-4939-9009-2_11&partnerID=40&md5=7a21261a5cf703a7eb3e8600b392b10f
Notes: Book Chapter 11 in "Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods. 2nd ed" (ISBN: 978-1-4939-9008-5) -- Source: Scopus
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MSK Authors
  1. Sonja Nowotschin
    37 Nowotschin
  2. Anna-Katerina Hadjantonakis
    191 Hadjantonakis
  3. Vidur Garg
    15 Garg
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