In vivo MRI of neural cell migration dynamics in the mouse brain Journal Article
| Authors: | Nieman, B. J.; Shyu, J. Y.; Rodriguez, J. J.; Garcia, A. Denise; Joyner, A. L.; Turnbull, D. H. |
| Article Title: | In vivo MRI of neural cell migration dynamics in the mouse brain |
| Abstract: | Multipotent neuroblasts (NBs) are produced throughout life by neural stem cells in the forebrain subventricular zone (SVZ), and are able to travel long distances to the olfactory bulb. On arrival in the bulb, migrating NBs normally replace olfactory neurons, raising interest in their potential for novel cell replacement therapies in various disease conditions. An understanding of the migratory capabilities of NBs is therefore important, but as yet quantitative in vivo measurement of cell migration has not been possible. In this study, targeted intracerebral injections of iron-oxide particles to the mouse SVZ were used to label resident NBs in situ, and their migration was tracked noninvasively over time with magnetic resonance imaging (MRI). Quantitative intensity metrics were employed to identify labeled cells and to show that cells are able to travel at speeds up to 100 μm/h en route to the olfactory bulb, but that distribution through the olfactory bulb occurs at a much slower rate. In addition, comparison of histological and MRI measures of iron-oxide particle distribution were in excellent agreement. Immunohistochemistry analysis 1-3 weeks after labeling revealed that the majority of labeled cells in the olfactory bulb were immature neurons, although iron-oxide particles were also found in astrocytes and microglia. This work indicates that dynamic measurements of endogenous cell migration can be made with MRI and represents the first in vivo measurement of NB migration rates. The use of MRI in future studies tracking endogenous NB cells will permit a more complete evaluation of their role during homeostasis at various developmental stages and during disease progression. © 2009 Elsevier Inc. All rights reserved. |
| Keywords: | immunohistochemistry; controlled study; nonhuman; nuclear magnetic resonance imaging; magnetic resonance imaging; animal cell; mouse; animal; cytology; animals; mice; animal tissue; image analysis; astrocyte; cell motion; in vivo study; subventricular zone; neurons; physiology; stem cell; feasibility study; brain; quantitative analysis; cell migration; cell movement; stem cells; contrast medium; contrast media; image processing, computer-assisted; image processing; neural stem cells; svz; nerve cell; neuroblast; olfactory bulb; cell labeling; ferric ion; ferric oxide; ferric compounds; microglia; micron-sized particles of iron-oxide; mpio; iron oxide; cryoprotection |
| Journal Title: | NeuroImage |
| Volume: | 50 |
| Issue: | 2 |
| ISSN: | 1053-8119 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2010-04-01 |
| Start Page: | 456 |
| End Page: | 464 |
| Language: | English |
| DOI: | 10.1016/j.neuroimage.2009.12.107 |
| PUBMED: | 20053381 |
| PROVIDER: | scopus |
| PMCID: | PMC2899477 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "CODEN: NEIME" - "Source: Scopus" |
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