PTEN in neural precursor cells: Regulation of migration, apoptosis, and proliferation Journal Article
| Authors: | Li, L.; Liu, F.; Salmonsen, R. A.; Turner, T. K.; Litofsky, N. S.; Di Cristofano, A.; Pandolfi, P. P.; Jones, S. N.; Recht, L. D.; Ross, A. H. |
| Article Title: | PTEN in neural precursor cells: Regulation of migration, apoptosis, and proliferation |
| Abstract: | PTEN is a lipid phosphatase, and PTEN mutations are associated with gliomas, macrocephaly, and mental deficiencies. We have used PTEN +/- mice to assess PTEN's role in subventricular zone (SVZ) precursor cells. For cultured SVZ neurosphere cells, haploinsufficiency for PTEN increases phosphorylation of Akt and forkhead transcription factor and slightly enhances proliferation. Based on a filter penetration assay, PTEN +/- cells are substantially more migratory and invasive than +/+ cells. The +/- cells also are more resistant to H2O2-induced apoptosis. Analysis of PTEN +/- and +/+ mice by BrdU labeling reveals no difference in the rate of cell proliferation in the SVZ. Exit of BrdU-labeled cells from the SVZ and radial migration to the outer layers of the olfactory bulb are more rapid for +/- cells. These observations indicate that PTEN regulates SVZ precursor cell function and is particularly important for migration and apoptosis in response to oxidative stress. |
| Keywords: | controlled study; protein phosphorylation; proto-oncogene proteins; nonhuman; glioma; cell proliferation; forkhead transcription factors; animal cell; mouse; animals; mice; mice, knockout; cell division; apoptosis; neurons; animalia; transcription factors; stem cell; regulatory mechanism; cell culture; brain; protein-serine-threonine kinases; tumor suppressor proteins; western blotting; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; proto-oncogene proteins c-akt; pten phosphohydrolase; cell migration; cell movement; cellular distribution; stem cells; cell count; oxidative stress; cell invasion; phosphoric monoester hydrolases; olfactory bulb; broxuridine; bromodeoxyuridine; cerebral ventricles; macrocephaly; mental deficiency; cell kinetics; male; female; priority journal; article; phosphatidylinositol phosphates |
| Journal Title: | Molecular and Cellular Neuroscience |
| Volume: | 20 |
| Issue: | 1 |
| ISSN: | 1044-7431 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2002-05-01 |
| Start Page: | 21 |
| End Page: | 29 |
| Language: | English |
| DOI: | 10.1006/mcne.2002.1115 |
| PUBMED: | 12056837 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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