Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres Journal Article
| Authors: | Ocbina, P. J.; Dizon, M. L. V.; Shin, L.; Szele, F. G. |
| Article Title: | Doublecortin is necessary for the migration of adult subventricular zone cells from neurospheres |
| Abstract: | Mutations in human doublecortin (DCX) and knockdown of Dcx in rodents cause radial migration defects in the embryonic cerebral cortex. However, the brain phenotype of Dcx knockout mice is largely normal suggesting that Dcx is not necessary for most migration events. Adult subventricular zone (SVZ) cells migrate tangentially in the rostral migratory stream to the olfactory bulbs. Dcx is expressed in the SVZ but it is unknown if it is necessary for migration. We show that Dcx RNAi reduced SVZ cell migration in vitro, both cell autonomously and non-cell autonomously. Overexpression of Dcx rescued migration after knockdown, but did not increase migration by itself. Thus, Dcx is necessary not only for embryonic radial migration but also migration of adult SVZ cells. © 2006 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; protein expression; mutation; nonhuman; protein function; animal cell; mouse; phenotype; animals; mice; mice, knockout; animal experiment; brain cortex; rna interference; age factors; in vitro study; hela cells; brain; brain ventricle; rna, messenger; brain development; nucleotide sequence; cell migration; cell movement; rodentia; cerebral cortex; cytoskeleton; neuropeptides; migration; knockout mouse; olfactory bulb; embryo cell; 3' untranslated regions; spheroids, cellular; microtubule-associated proteins; brain cell; doublecortin; neurospheres; subependymal zone; videomicroscopy; lateral ventricles; microscopy, video |
| Journal Title: | Molecular and Cellular Neuroscience |
| Volume: | 33 |
| Issue: | 2 |
| ISSN: | 1044-7431 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2006-10-09 |
| Start Page: | 126 |
| End Page: | 135 |
| Language: | English |
| DOI: | 10.1016/j.mcn.2006.06.014 |
| PUBMED: | 16931042 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 22" - "Export Date: 4 June 2012" - "CODEN: MOCNE" - "Molecular Sequence Numbers: GENBANK: BC056391;" - "Source: Scopus" |
