High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice Journal Article

  

Authors:	Mayer-Kuckuk, P.; Gade, T. P. F.; Buchanan, I. M.; Doubrovin, M.; Ageyeva, L.; Bertino, J. R.; Boskey, A. L.; Blasberg, R. G.; Koutcher, J. A.; Banerjee, D.
Article Title:	High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice
Abstract:	Bone precursor cells (BPCs) play a critical role in bone maintenance and regeneration. Currently, no tool exists to study BPCs or other bone marrow cell types directly within their complex microenvironment. Here, we describe in vivo magnetic resonance imaging (MRI) of anatomical structures inside the medullary cavity of the mouse femur. We demonstrate that BPCs passively labeled with iron oxide-containing particles can be monitored by MRI within the intact bone marrow at an in-plane resolution of 43 × 25 μm. Anatomical detail provided by MRI is complemented by functional optical imaging of reporter gene expression. Single-cell dual iron oxide-reporter gene labeling has potential for combined cell tracking and cell biology studies. In summary, we describe a versatile platform suitable for studying the biology of many bone marrow cell types in living bone. Copyright © The American Society of Gene Therapy.
Keywords:	controlled study; nonhuman; nuclear magnetic resonance imaging; magnetic resonance imaging; animal cell; mouse; cytology; animals; mice; bone marrow cells; animal experiment; molecular imaging; mice, inbred c57bl; cell type; luminescent proteins; transduction, genetic; reporter gene; mesenchymal stem cell; genes, reporter; bone marrow cell; hematopoietic stem cell; stroma cell; femur; non invasive measurement; bioluminescence; cell transplantation; cell tracking; cell labeling; staining and labeling; bone regeneration; bioluminescence imaging; superparamagnetic iron oxide; marrow stromal cells; bone precursor cell
Journal Title:	Molecular Therapy
Volume:	12
Issue:	1
ISSN:	1525-0016
Publisher:	Nature Publishing Group
Date Published:	2005-07-01
Start Page:	33
End Page:	41
Language:	English
DOI:	10.1016/j.ymthe.2005.02.018
PUBMED:	15963918
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-20844435026&partnerID=40&md5=22813640317a18ea1220880ba72dc91d
Notes:	--- - "Cited By (since 1996): 5" - "Export Date: 24 October 2012" - "CODEN: MTOHC" - "Source: Scopus"

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