Molecular imaging reveals skeletal engraftment sites of transplanted bone marrow cells Journal Article
| Authors: | Mayer-Kuckuk, P.; Doubrovin, M.; Bidaut, L.; Budak-Alpdogan, T.; Cai, S.; Hubbard, V.; Alpdogan, O.; Van Den Brink, M.; Bertino, J. R.; Blasberg, R. G.; Banerjee, D.; Gelovani, J. |
| Article Title: | Molecular imaging reveals skeletal engraftment sites of transplanted bone marrow cells |
| Abstract: | Molecular imaging holds great promise for the in vivo study of cell therapy. Our hypothesis was that multimodality molecular imaging can identify the initial skeletal engraftment sites post-bone marrow cell transplantation. Utilizing a standard mouse model of bone marrow (BM) transplantation, we introduced a combined bioluminescence (BLI) and positron emission tomography (PET) imaging reporter gene into mouse bone marrow cells. Bioluminescence imaging was used for monitoring serially the early in vivo BM cell engraftment/expansion every 24 h. Significant cell engraftment/expansion was noted by greatly increased bioluminescence about 1 week posttransplant. Then PET was applied to acquire three-dimensional images of the whole-body in vivo biodistribution of the transplanted cells. To localize cells in the skeleton, PET was followed by computed tomography (CT). Co-registration of PET and CT mapped the sites of BM engraftment. Multiple, discrete BM cell engraftment sites were observed. Taken together, this multimodality approach may be useful for further in vivo characterization of various therapeutic cell types. Copyright © 2006 Cognizant Comm. Corp. |
| Keywords: | nonhuman; positron emission tomography; animal cell; mouse; animals; mice; bone marrow cells; computer assisted tomography; image analysis; animal experiment; tomography, x-ray computed; molecular imaging; transplantation; mice, inbred c57bl; animalia; transduction, genetic; models, animal; luminescent measurements; three dimensional imaging; imaging, three-dimensional; positron-emission tomography; reporter gene; cellular distribution; ligands; genes, reporter; bone and bones; mouse strain; bone marrow transplantation; signal detection; bioluminescence; cell transplantation; positron emission tomography (pet); retroviridae; skeleton; computed tomography (ct); bioluminescence imaging |
| Journal Title: | Cell Transplantation |
| Volume: | 15 |
| Issue: | 1 |
| ISSN: | 0963-6897 |
| Publisher: | Cognizant Communication Corporation |
| Date Published: | 2006-01-01 |
| Start Page: | 75 |
| End Page: | 82 |
| Language: | English |
| PUBMED: | 16700332 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 10" - "Export Date: 4 June 2012" - "CODEN: CTRAE" - "Source: Scopus" |
Citation Impact
MSK Authors
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136Banerjee -
7
Bidaut -
272Blasberg -
616Van Den Brink -
75
Alpdogan -
42Cai -
18
Mayer-Kuckuk -
42
Hubbard -
97
Doubrovin
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