Copper-64 labeled liposomes for imaging bone marrow Journal Article

   


Authors: Lee, S. G.; Gangangari, K.; Kalidindi, T. M.; Punzalan, B.; Larson, S. M.; Pillarsetty, N. V. K.
Article Title: Copper-64 labeled liposomes for imaging bone marrow
Abstract: Introduction Bone marrow is the soft tissue compartment inside the bones made up of hematopoietic cells, adipocytes, stromal cells, phagocytic cells, stem cells, and sinusoids. While [18F]-FLT has been utilized to image proliferative marrow, to date, there are no reports of particle based positron emission tomography (PET) imaging agents for imaging bone marrow. We have developed copper-64 labeled liposomal formulation that selectively targets bone marrow and therefore serves as an efficient PET probe for imaging bone marrow. Methods Optimized liposomal formulations were prepared with succinyl PE, DSPC, cholesterol, and mPEG-DSPE (69:39:1:10:0.1) with diameters of 90 and 140 nm, and were doped with DOTA-Bn-DSPE for stable 64Cu incorporation into liposomes. Results PET imaging and biodistribution studies with 64Cu-labeled liposomes indicate that accumulation in bone marrow was as high as 15.18 ± 3.69%ID/g for 90 nm liposomes and 7.01 ± 0.92%ID/g for 140 nm liposomes at 24 h post-administration. In vivo biodistribution studies in tumor-bearing mice indicate that the uptake of 90 nm particles is approximately 0.89 ± 0.48%ID/g in tumor and 14.22 ± 8.07%ID/g in bone marrow, but respective values for Doxil® like liposomes are 0.83 ± 0.49%ID/g and 2.23 ± 1.00%ID/g. Conclusion Our results indicate that our novel PET labeled liposomes target bone marrow with very high efficiency and therefore can function as efficient bone marrow imaging agents. © 2016 Elsevier Inc.
Keywords: bone marrow; pet; liposome; copper-64; dota-bn-dspe
Journal Title: Nuclear Medicine and Biology
Volume: 43
Issue: 12
ISSN: 0969-8051
Publisher: Elsevier Science Inc.  
Date Published: 2016-12-01
Start Page: 781
End Page: 787
Language: English
DOI: 10.1016/j.nucmedbio.2016.08.011
PROVIDER: scopus
PUBMED: 27694056
PMCID: PMC5118102
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84989202989&partnerID=40&md5=a9110c2d128b563090a01d317824dae4
Notes: Article -- Export Date: 2 November 2016 -- Source: Scopus
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MSK Authors
  1. Nagavarakishore Pillarsetty
    133 Pillarsetty
  2. Steven M Larson
    959 Larson
  3. Blesida Punzalan
    38 Punzalan
  4. Sang Gyu Lee
    21 Lee
  5. Teja Muralidhar Kalidindi
    39 Kalidindi
  6. Kishore Kumar Gangangari
    11 Gangangari
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