Myosin II dynamics are regulated by tension in intercalating cells Journal Article

Authors: Fernandez-Gonzalez, R.; Simoes, S. d M.; Röper, J. C.; Eaton, S.; Zallen, J. A.
Article Title: Myosin II dynamics are regulated by tension in intercalating cells
Abstract: Axis elongation in Drosophila occurs through polarized cell rearrangements driven by actomyosin contractility. Myosin II promotes neighbor exchange through the contraction of single cell boundaries, while the contraction of myosin II structures spanning multiple pairs of cells leads to rosette formation. Here we show that multicellular actomyosin cables form at a higher frequency than expected by chance, indicating that cable assembly is an active process. Multicellular cables are sites of increased mechanical tension as measured by laser ablation. Fluorescence recovery after photobleaching experiments show that myosin II is stabilized at the cortex in regions of increased tension. Myosin II is recruited in response to an ectopic force and relieving tension leads to a rapid loss of myosin, indicating that tension is necessary and sufficient for cortical myosin localization. These results demonstrate that myosin II dynamics are regulated by tension in a positive feedback loop that leads to multicellular actomyosin cable formation and efficient tissue elongation. © 2009 Elsevier Inc. All rights reserved.
Keywords: controlled study; nonhuman; protein localization; animal cell; animals; animal tissue; embryo; protein depletion; bleaching; protein stability; embryo, nonmammalian; gene expression regulation, developmental; cellbio; devbio; drosophila melanogaster; laser surgery; myosin adenosine triphosphatase; myosin ii; cell elongation; fluorescence analysis; muscle contractility; positive feedback; tension; actomyosin; muscle tonus; myosin type ii
Journal Title: Developmental Cell
Volume: 17
Issue: 5
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2009-11-17
Start Page: 736
End Page: 743
Language: English
DOI: 10.1016/j.devcel.2009.09.003
PUBMED: 19879198
PROVIDER: scopus
PMCID: PMC2854079
Notes: --- - "Cited By (since 1996): 15" - "Export Date: 30 November 2010" - "CODEN: DCEEB" - "Source: Scopus"
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MSK Authors
  1. Jennifer A Zallen
    39 Zallen
  2. Sergio Manuel De Matos Simoes
    4 Simoes