Integration of contractile forces during tissue invagination Journal Article
| Authors: | Martin, A. C.; Gelbart, M.; Fernandez-Gonzalez, R.; Kaschube, M.; Wieschaus, E. F. |
| Article Title: | Integration of contractile forces during tissue invagination |
| Abstract: | Contractile forces generated by the actomyosin cytoskeleton within individual cells collectively generate tissue-level force during epithelial morphogenesis. During Drosophila mesoderm invagination, pulsed actomyosin meshwork contractions and a ratchetlike stabilization of cell shape drive apical constriction. Here, we investigate how contractile forces are integrated across the tissue. Reducing adherens junction (AJ) levels or ablating actomyosin meshworks causes tissue-wide epithelial tears, which release tension that is predominantly oriented along the anterior-posterior (a-p) embryonic axis. Epithelial tears allow cells normally elongated along the a-p axis to constrict isotropically, which suggests that apical constriction generates anisotropic epithelial tension that feeds back to control cell shape. Epithelial tension requires the transcription factor Twist, which stabilizes apical myosin II, promoting the formation of a supracellular actomyosin meshwork in which radial actomyosin fibers are joined end-to-end at spot AJs. Thus, pulsed actomyosin contractions require a supracellular, tensile meshwork to transmit cellular forces to the tissue level during morphogenesis. © 2010 Martin et al. |
| Keywords: | nonhuman; biological markers; animals; drosophila; morphogenesis; rna interference; transcription factors; recombinant fusion proteins; cell membrane; drosophila melanogaster; epithelium; cell shape; cell junction; cell adhesion; myosin adenosine triphosphatase; myosin ii; actomyosin; myosin type ii; transcription factor twist; cytoskeleton; drosophila proteins; anisotropy; adherens junctions; invagination; animals, genetically modified; embryo axis; stress, mechanical; twist transcription factor |
| Journal Title: | Journal of Cell Biology |
| Volume: | 188 |
| Issue: | 5 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2010-03-08 |
| Start Page: | 735 |
| End Page: | 749 |
| Language: | English |
| DOI: | 10.1083/jcb.200910099 |
| PUBMED: | 20194639 |
| PROVIDER: | scopus |
| PMCID: | PMC2835944 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 10" - "Export Date: 20 April 2011" - "CODEN: JCLBA" - "Source: Scopus" |
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