Methods for the study of entosis Journal Article
| Authors: | Sun, Q.; Overholtzer, M. |
| Article Title: | Methods for the study of entosis |
| Abstract: | Entosis is a recently described nonapoptotic cell death mechanism that is initiated by the engulfment of live epithelial cells, leading to the formation of "cell-in-cell" structures. Entotic cell engulfment is induced by matrix detachment, and is driven by imbalances in actomyosin contraction between neighboring cells. Here we describe methods to quantify the formation of cell-in-cell structures by entosis, for cells cultured in suspension or in soft agar, by fluorescence imaging and time-lapse microscopy. © Springer Science and Business Media, LLC 2013. |
| Keywords: | microscopy; cell structure; cell culture; suspension cell culture; fluorescence imaging; time-lapse; entosis; cannibalism; engulfment; anoikis; cell-in-cell; soft agar; agar medium; time lapse microscopy |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1004 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2013-01-01 |
| Start Page: | 59 |
| End Page: | 66 |
| Language: | English |
| DOI: | 10.1007/978-1-62703-383-1_5 |
| PROVIDER: | scopus |
| PUBMED: | 23733569 |
| DOI/URL: | |
| Notes: | Chapter 5 in "Necrosis: Methods and Protocols" (ISBN: 978-1-62703-382-4) --- Export Date: 1 October 2013 - Source: Scopus |
