H2O2: A chemoattractant? Journal Article

Authors: Enyedi, B.; Niethammer, P.
Article Title: H2O2: A chemoattractant?
Abstract: H2O2 is a relatively stable, rapidly diffusing reactive oxygen species that has been recently implicated as a mediator of leukocyte recruitment to epithelial wounds and transformed cells in zebrafish. Whether H2O2 activates the innate immune response by acting as a bona fide chemoattractant, enhancing chemoattractant sensing, or triggering production of other chemoattractive ligands remains largely unclear. Here, we describe the basic experimental procedures required to study these questions. We present a detailed protocol of the zebrafish tail fin wounding assay and explain how to use it for analyzing leukocyte chemotaxis in vivo. We further outline a method for H2O2 measurement in live zebrafish larvae using the genetically encoded sensor HyPer on a wide-field and a spinning disk confocal microscope. These methods provide a basis for dissecting the role of H2O2 in leukocyte chemotaxis in a vertebrate animal. © 2013 Elsevier Inc.
Keywords: chemotaxis; leukocyte; h2o2; duox; hyper; zebrafish wound
Journal Title: Methods in Enzymology
Volume: 528
ISSN: 0076-6879
Publisher: Academic Press  
Date Published: 2013-01-01
Start Page: 237
End Page: 255
Language: English
DOI: 10.1016/b978-0-12-405881-1.00014-8
PROVIDER: scopus
PUBMED: 23849869
Notes: Chapter 14 in "Hydrogen Peroxide and Cell Signaling, Part C" (ISBN: 978-0-12-405881-1) - "Export Date: 1 August 2013" - "CODEN: MENZA" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Balazs Gabor Enyedi
    10 Enyedi