Osmotic surveillance mediates rapid wound closure through nucleotide release Journal Article


Authors: Gault, W. J.; Enyedi, B.; Niethammer, P.
Article Title: Osmotic surveillance mediates rapid wound closure through nucleotide release
Abstract: Osmotic cues from the environment mediate rapid detection of epithelial breaches by leukocytes in larval zebrafish tail fins. Using intravital luminescence and fluorescence microscopy, we now show that osmolarity differences between the interstitial fluid and the external environment trigger ATP release at tail fin wounds to initiate rapid wound closure through long-range activation of basal epithelial cell motility. Extracellular nucleotide breakdown, at least in part mediated by ecto-nucleoside triphosphate diphosphohydrolase 3 (Entpd3), restricts the range and duration of osmotically induced cell migration after injury. Thus, in zebrafish larvae, wound repair is driven by an autoregulatory circuit that generates pro-migratory tissue signals as a function of environmental exposure of the inside of the tissue. © 2014 Gault et al.
Keywords: adult; controlled study; nonhuman; ultraviolet radiation; animal cell; in vitro study; luminescence; plasmid; cell migration; protein secretion; epithelium cell; fluorescence microscopy; adenosine triphosphate; cell motility; hydrolysis; wound closure; environmental exposure; danio rerio; larva; nucleoside triphosphate; muscle hypotonia; transgenic zebrafish; priority journal; article; epithelium basal cell; nucleotide release; osmotic pressure; particle image velocimetry; rapid epithelial wound closure
Journal Title: Journal of Cell Biology
Volume: 207
Issue: 6
ISSN: 0021-9525
Publisher: Rockefeller University Press  
Date Published: 2014-12-22
Start Page: 767
End Page: 782
Language: English
DOI: 10.1083/jcb.201408049
PROVIDER: scopus
PMCID: PMC4274268
PUBMED: 25533845
DOI/URL:
Notes: Export Date: 2 July 2015 -- Source: Scopus
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MSK Authors
  1. William Gault
    2 Gault
  2. Balazs Gabor Enyedi
    9 Enyedi