Authors: | Jelcic, M.; Enyedi, B.; Niethammer, P. |
Article Title: | Quantitative imaging of endogenous and exogenous H(2)O(2) gradients in live Zebrafish larvae |
Abstract: | Quantitative aspects of extracellular H2O2 signaling in animals, such as its spatiotemporal dynamics within tissues, remain little understood. Here we detail an optimized, experimental setup for measuring the dynamics and physiological consequences of extracellular H2O2 application to live tissues by intravital biosensor imaging in zebrafish larvae. © Springer Science+Business Media, LLC, part of Springer Nature 2019. |
Keywords: | diffusion; quantitative; hydrogen peroxide; zebrafish; gradient; biosensor; intravital |
Journal Title: | Methods in Molecular Biology |
Volume: | 1982 |
ISSN: | 1064-3745 |
Publisher: | Humana Press Inc |
Date Published: | 2019-01-01 |
Start Page: | 283 |
End Page: | 299 |
Language: | English |
DOI: | 10.1007/978-1-4939-9424-3_17 |
PUBMED: | 31172479 |
PROVIDER: | scopus |
DOI/URL: | |
Notes: | Chapter 17 in "NADPH Oxidases: Methods and Protocols" (ISBN: 978-1-4939-9423-6) -- Source: Scopus |