Hydrogen peroxide generated extracellularly by receptor-ligand interaction facilitates cell signaling Journal Article
| Authors: | DeYulia, G. J. Jr; Cárcamo, J. M.; Borquez-Ojeda, O.; Shelton, C. C.; Golde, D. W. |
| Article Title: | Hydrogen peroxide generated extracellularly by receptor-ligand interaction facilitates cell signaling |
| Abstract: | Reactive oxygen species (ROS) are key components of postreceptor intracellular signaling pathways; however, the role of ROS in signal initiation is uncertain. We discovered that receptor-ligand interaction caused the generation of hydrogen peroxide (H2O2). Using members of the hematopoietin receptor superfamily, as well as EGF receptor, we show that H2O2 is generated by specific receptor-ligand interaction in cells and in cell-free systems. With cognate ligand, the extracellular domain of the receptor was sufficient for H2O2 generation. We also found that production of H2O2 was diminished in a granulocyte-macrophage colony-stimulating factor receptor mutant unable to bind ligand. Exogenously added H2O2 induced signaling in the absence of ligand, whereas catalase and a membrane-bound peroxiredoxin inhibited ligand-dependent signaling. Our results suggest that H2O2 produced by receptor-ligand interaction is involved as a chemical mediator that facilitates cell signaling. © 2005 by The National Academy of Sciences of the USA. |
| Keywords: | signal transduction; epidermal growth factor; controlled study; human cell; mutation; models, biological; epidermal growth factor receptor; cell line; receptor, epidermal growth factor; enzyme activity; transfection; recombinant proteins; reactive oxygen species; reactive oxygen metabolite; ligands; base sequence; hydrogen peroxide; protein subunits; cell communication; extracellular fluid; ligand binding; receptor binding; erythropoietin receptor; dna, complementary; receptors, cell surface; kinase; granulocyte-macrophage colony-stimulating factor; catalase; granulocyte macrophage colony stimulating factor receptor; receptors, granulocyte-macrophage colony-stimulating factor; cytokine hematopoietin; peroxidases |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 102 |
| Issue: | 14 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2005-04-05 |
| Start Page: | 5044 |
| End Page: | 5049 |
| Language: | English |
| DOI: | 10.1073/pnas.0501154102 |
| PUBMED: | 15795385 |
| PROVIDER: | scopus |
| PMCID: | PMC556007 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 62" - "Export Date: 24 October 2012" - "CODEN: PNASA" - "Source: Scopus" |
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