Regulation of cytoskeletal organization and junctional remodeling by the atypical cadherin Fat Journal Article

Authors: Marcinkevicius, E.; Zallen, J. A.
Article Title: Regulation of cytoskeletal organization and junctional remodeling by the atypical cadherin Fat
Abstract: The atypical cadherin Fat is a conserved regulator of planar cell polarity, but the mechanisms by which Fat controls cell shape and tissue structure are not well understood. Here, we show that Fat is required for the planar polarized organization of actin denticle precursors, adherens junction proteins and microtubules in the epidermis of the late Drosophila embryo. In wild-type embryos, spatially regulated cell-shape changes and rearrangements organize cells into highly aligned columns. Junctional remodeling is suppressed at dorsal and ventral cell boundaries, where adherens junction proteins accumulate. By contrast, adherens junction proteins fail to accumulate to the wild-type extent and all cell boundaries are equally engaged in junctional remodeling in fat mutants. The effects of loss of Fat on cell shape and junctional localization, but not its role in denticle organization, are recapitulated by mutations in Expanded, an upstream regulator of the conserved Hippo pathway, and mutations in Hippo and Warts, two kinases in the Hippo kinase cascade. However, the cell shape and planar polarity defects in fat mutants are not suppressed by removing the transcriptional co-activator Yorkie, suggesting that these roles of Fat are independent of Yorkie-mediated transcription. The effects of Fat on cell shape, junctional remodeling and microtubule localization are recapitulated by expression of activated Notch. These results demonstrate that cell shape, junctional localization and cytoskeletal planar polarity in the Drosophila embryo are regulated by a common signal provided by the atypical cadherin Fat and suggest that Fat influences tissue organization through its role in polarized junctional remodeling. © 2013. Published by The Company of Biologists Ltd.
Keywords: unclassified drug; nonhuman; protein localization; animal cell; actin; epidermis; embryo; protein depletion; cell protein; notch receptor; drosophila; cell shape; cell junction; microtubule; microtubules; cadherin; cell adhesion; cytoskeleton; fat; frizzled protein; planar polarity; adherens junction protein; fat protein
Journal Title: Development
Volume: 140
Issue: 2
ISSN: 0950-1991
Publisher: Company of Biologists  
Date Published: 2013-01-15
Start Page: 433
End Page: 443
Language: English
DOI: 10.1242/dev.083949
PROVIDER: scopus
PUBMED: 23250217
PMCID: PMC3597213
Notes: --- - "Export Date: 1 February 2013" - "CODEN: DEVPE" - "Source: Scopus"
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MSK Authors
  1. Jennifer A Zallen
    39 Zallen