Abl regulates planar polarized junctional dynamics through β-catenin tyrosine phosphorylation Journal Article


Authors: Tamada, M.; Farrell, D. L.; Zallen, J. A.
Article Title: Abl regulates planar polarized junctional dynamics through β-catenin tyrosine phosphorylation
Abstract: Interactions between epithelial cells are mediated by adherens junctions that are dynamically regulated during development. Here we show that the turnover of β-catenin is increased at cell interfaces that are targeted for disassembly during Drosophila axis elongation. The Abl tyrosine kinase is concentrated at specific planar junctions and is necessary for polarized β-catenin localization and dynamics. abl mutant embryos have decreased β-catenin turnover at shrinking edges, and these defects are accompanied by a reduction in multicellular rosette formation and axis elongation. Abl promotes β-catenin phosphorylation on the conserved tyrosine 667 and expression of an unphosphorylatable β-catenin mutant recapitulates the defects of abl mutants. Notably, a phosphomimetic β-catenin Y667E mutation is sufficient to increase β-catenin turnover and rescue axis elongation in abl deficient embryos. These results demonstrate that the asymmetrically localized Abl tyrosine kinase directs planar polarized junctional remodeling during Drosophila axis elongation through the tyrosine phosphorylation of β-catenin. Epithelial morphogenesis in the Drosophila embryo requires multicellular junctional remodeling. Tamada etal. show that asymmetrically localized Abl tyrosine kinase generates spatially regulated adherens junction dynamics through the tyrosine phosphorylation of β-catenin. Abl activity and polarized junctional dynamics are necessary for formation of multicellular rosette and Drosophila body axis elongation. © 2012 Elsevier Inc.
Keywords: controlled study; protein phosphorylation; nonhuman; protein localization; animal cell; animal tissue; embryo; protein assembly; molecular dynamics; drosophila; in vivo study; abelson kinase; tyrosine; nucleotide sequence; cell polarity; rosette formation; cell junction; protein structure; beta catenin; mutant
Journal Title: Developmental Cell
Volume: 22
Issue: 2
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2012-02-14
Start Page: 309
End Page: 319
Language: English
DOI: 10.1016/j.devcel.2011.12.025
PROVIDER: scopus
PUBMED: 22340496
PMCID: PMC3327890
DOI/URL:
Notes: --- - "Export Date: 2 April 2012" - "CODEN: DCEEB" - "Source: Scopus"
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MSK Authors
  1. Jennifer A Zallen
    39 Zallen
  2. Masako Tamada
    6 Tamada