Rho-kinase directs bazooka/Par-3 planar polarity during drosophila axis elongation Journal Article


Authors: Simoes, S. d M.; Blankenship, J. T.; Weitz, O.; Farrell, D. L.; Tamada, M.; Fernandez-Gonzalez, R.; Zallen, J. A.
Article Title: Rho-kinase directs bazooka/Par-3 planar polarity during drosophila axis elongation
Abstract: Cell rearrangements shape the Drosophila embryo via spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of the Baz domain, and activated Rho-kinase is sufficient to exclude Baz from the cortex. The planar polarized distribution of Baz requires its C-terminal domain. Rho-kinase can phosphorylate this domain and inhibit its interaction with phosphoinositide membrane lipids, suggesting a mechanism by which Rho-kinase could regulate Baz association with the cell cortex. These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains. © 2010 Elsevier Inc.
Keywords: immunohistochemistry; controlled study; unclassified drug; nonhuman; protein domain; animals; animal tissue; carboxy terminal sequence; embryo; drosophila; enzyme activation; phosphorylation; embryo, nonmammalian; gene expression regulation, developmental; immunoenzyme techniques; blotting, western; enzyme phosphorylation; enzyme regulation; reverse transcriptase polymerase chain reaction; rna, messenger; intracellular signaling peptides and proteins; cell polarity; protein kinase c; down regulation; drosophila melanogaster; cell nucleus; cell junction; myosin; myosin type ii; drosophila proteins; body patterning; transgenes; rho-associated kinases; rho kinase; animals, genetically modified; myosin 2; phosphatidylinositide; proteinase activated receptor 3
Journal Title: Developmental Cell
Volume: 19
Issue: 3
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2010-09-14
Start Page: 377
End Page: 388
Language: English
DOI: 10.1016/j.devcel.2010.08.011
PUBMED: 20833361
PROVIDER: scopus
PMCID: PMC3131216
DOI/URL:
Notes: --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "CODEN: DCEEB" - "Source: Scopus"
Altmetric Score