Rho-kinase directs bazooka/Par-3 planar polarity during drosophila axis elongation Journal Article
| Authors: | Simoes, S. d M.; Blankenship, J. T.; Weitz, O.; Farrell, D. L.; Tamada, M.; Fernandez-Gonzalez, R.; Zallen, J. A. |
| Article Title: | Rho-kinase directs bazooka/Par-3 planar polarity during drosophila axis elongation |
| Abstract: | Cell rearrangements shape the Drosophila embryo via spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at the anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of the Baz domain, and activated Rho-kinase is sufficient to exclude Baz from the cortex. The planar polarized distribution of Baz requires its C-terminal domain. Rho-kinase can phosphorylate this domain and inhibit its interaction with phosphoinositide membrane lipids, suggesting a mechanism by which Rho-kinase could regulate Baz association with the cell cortex. These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains. © 2010 Elsevier Inc. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; nonhuman; protein domain; animals; animal tissue; carboxy terminal sequence; embryo; drosophila; enzyme activation; phosphorylation; embryo, nonmammalian; gene expression regulation, developmental; immunoenzyme techniques; blotting, western; enzyme phosphorylation; enzyme regulation; reverse transcriptase polymerase chain reaction; rna, messenger; intracellular signaling peptides and proteins; cell polarity; protein kinase c; down regulation; drosophila melanogaster; cell nucleus; cell junction; myosin; myosin type ii; drosophila proteins; body patterning; transgenes; rho-associated kinases; rho kinase; animals, genetically modified; myosin 2; phosphatidylinositide; proteinase activated receptor 3 |
| Journal Title: | Developmental Cell |
| Volume: | 19 |
| Issue: | 3 |
| ISSN: | 1534-5807 |
| Publisher: | Cell Press |
| Date Published: | 2010-09-14 |
| Start Page: | 377 |
| End Page: | 388 |
| Language: | English |
| DOI: | 10.1016/j.devcel.2010.08.011 |
| PUBMED: | 20833361 |
| PROVIDER: | scopus |
| PMCID: | PMC3131216 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "CODEN: DCEEB" - "Source: Scopus" |
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