A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate Journal Article

   

Authors:	Brooks, E. R.; Moorman, A. R.; Bhattacharya, B.; Prudhomme, I. S.; Land, M.; Alcorn, H. L.; Sharma, R.; Pe'er, D.; Zallen, J. A.
Article Title:	A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate
Abstract:	The formation of the mammalian brain requires regionalization and morphogenesis of the cranial neural plate, which transforms from an epithelial sheet into a closed tube that provides the structural foundation for neural patterning and circuit formation. Sonic hedgehog (SHH) signaling is important for cranial neural plate patterning and closure, but the transcriptional changes that give rise to the spatially regulated cell fates and behaviors that build the cranial neural tube have not been systematically analyzed. Here, we used single-cell RNA sequencing to generate an atlas of gene expression at six consecutive stages of cranial neural tube closure in the mouse embryo. Ordering transcriptional profiles relative to the major axes of gene expression predicted spatially regulated expression of 870 genes along the anterior-posterior and mediolateral axes of the cranial neural plate and reproduced known expression patterns with over 85% accuracy. Single-cell RNA sequencing of embryos with activated SHH signaling revealed distinct SHH-regulated transcriptional programs in the developing forebrain, midbrain, and hindbrain, suggesting a complex interplay between anterior-posterior and mediolateral patterning systems. These results define a spatiotemporally resolved map of gene expression during cranial neural tube closure and provide a resource for investigating the transcriptional events that drive early mammalian brain development.
Keywords:	transcription factor; brain development; retinoic acid; mouse embryo; patterning; homeobox gene; neural plate; neural tube closure; signaling pathway; inner-ear; developmental defects; sonic hedgehog pathway; tube defects; head morphogenesis; single-cell rna sequencing; anterior neuroectoderm
Journal Title:	eLife
Volume:	13
ISSN:	2050-084X
Publisher:	eLife Sciences Publications Ltd.
Date Published:	2025-04-07
Start Page:	RP102819
Language:	English
ACCESSION:	WOS:001462305200001
DOI:	10.7554/eLife.102819
PROVIDER:	Clarivate Analytics Web of Science
PROVIDER:	wos
PMCID:	PMC11975377
PUBMED:	40192104
Notes:	Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Jennifer Zallen -- Source: Wos

https://synapse.mskcc.org/synapse/works/has_related_data_chk/224980