Sonic hedgehog signaling directs patterned cell remodeling during cranial neural tube closure Journal Article

   

Authors:	Brooks, E. R.; Islam, M. T.; Anderson, K. V.; Zallen, J. A.
Article Title:	Sonic hedgehog signaling directs patterned cell remodeling during cranial neural tube closure
Abstract:	Neural tube closure defects are a major cause of infant mortality, with exencephaly accounting for nearly one-third of cases. However, the mechanisms of cranial neural tube closure are not well understood. Here we show that this process involves a tissue-wide pattern of apical constriction controlled by Sonic hedgehog (Shh) signaling. Midline cells in the mouse midbrain neuroepithelium are short with large apical surfaces, whereas lateral cells are taller and undergo synchronous apical constriction, driving neural fold elevation. Embryos lacking the Shh effector Gli2 fail to produce appropriate midline cell architecture, whereas embryos with expanded Shh signaling, including the IFT-A complex mutants Ift122 and Ttc21b and embryos expressing activated Smoothened, display apical constriction defects in lateral cells. Disruption of lateral, but not midline, cell remodeling results in exencephaly. These results reveal a morphogenetic program of patterned apical constriction governed by Shh signaling that generates structural changes in the developing mammalian brain. © 2020, eLife Sciences Publications Ltd. All rights reserved.
Journal Title:	eLife
Volume:	9
ISSN:	2050-084X
Publisher:	eLife Sciences Publications Ltd.
Date Published:	2020-10-26
Start Page:	e60234
Language:	English
DOI:	10.7554/eLife.60234
PUBMED:	33103996
PROVIDER:	scopus
PMCID:	PMC7655103
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095604585&doi=10.7554%2feLife.60234&partnerID=40&md5=39a0c56a07dbd51d3abef213f28bab6d
Notes:	Article -- Export Date: 1 December 2020 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/173151