Dorsal and lateral fates in the mouse neural tube require the cell-autonomous activity of the open brain gene Journal Article
| Authors: | Eggenschwiler, J. T.; Anderson, K. V. |
| Article Title: | Dorsal and lateral fates in the mouse neural tube require the cell-autonomous activity of the open brain gene |
| Abstract: | The processes that specify early regional identity in dorsal and lateral regions of the mammalian neural tube are not well understood. The mouse open brain (opb) gene plays an essential role in dorsal neural patterning: in the caudal spinal cord of opb mutants, dorsal cell types are absent and markers of ventral fates, including Shh, expand into dorsal regions. Analysis of the opb mutant phenotype and of opb/opb mutually implies wild-type chimeric embryos reveals that early in neural development, the wild-type opb gene (opb+) is required cell autonomously for the expression of Pax7 in dorsal cells and Pax6 in lateral cells. Thus the opb+ gene product acts intracellularly in the reception or interpretation of signals that determine cell types in the dorsal 80% of the neural tube. At later stages, the lack of opb+ causes a non-cell-autonomous expansion of ventral cell types into dorsal regions of the neural tube, revealing that opb+ controls the production of a diffusible molecule that defines the domain of Shh expression. The data indicate that opb+ could act as either a novel component of a dorsalizing pathway or a novel intracellular negative regulator of the Shh signal transduction pathway. (C) 2000 Academic Press. |
| Keywords: | signal transduction; controlled study; protein expression; unclassified drug; gene mutation; mutation; dna-binding proteins; nonhuman; proteins; animal cell; mouse; mammalia; animals; mice; animal tissue; genes; embryo; sonic hedgehog protein; animal experiment; hedgehog proteins; mice, mutant strains; protein; gene product; cell fate; morphogenesis; eye proteins; homeodomain proteins; mice, inbred c57bl; central nervous system; cell type; animalia; transcription factors; chimera; mammal; spinal cord; gene control; trans-activators; transcription factor pax6; cell marker; repressor proteins; sonic hedgehog; erinaceidae; body patterning; neural tube; paired box transcription factors; gene activity; bone morphogenetic proteins; mice, inbred c3h; beta-galactosidase; models, neurological; transcription factor pax7; pax7 transcription factor; dorsal-ventral patterning; priority journal; article; open brain; msx1 transcription factor |
| Journal Title: | Developmental Biology |
| Volume: | 227 |
| Issue: | 2 |
| ISSN: | 0012-1606 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2000-11-15 |
| Start Page: | 648 |
| End Page: | 660 |
| Language: | English |
| DOI: | 10.1006/dbio.2000.9918 |
| PUBMED: | 11071781 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
