Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo Journal Article


Authors: Garcia-Garcia, M. J.; Shibata, M.; Anderson, K. V.
Article Title: Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo
Abstract: In Xenopus and zebrafish embryos, elongation of the anterior-posterior body axis depends on convergent extension, a process that involves polarized cell movements and is regulated by non-canonical Wnt signaling. The mechanisms that control axis elongation of the mouse embryo are much less well understood. Here, we characterize the ENU-induced mouse mutation chato, which causes arrest at midgestation and defects characteristic of convergent extension mutants, including a shortened body axis, mediolaterally extended somites and an open neural tube. The chato mutation disrupts Zfp568, a Krüppel-associated box (KRAB) domain zincfinger protein. Morphometric analysis revealed that the definitive endoderm of mouse wild-type embryos undergoes cell rearrangements that lead to convergent extension during early somite stages, and that these cell rearrangements fail in chato embryos. Although non-canonical Wnt signaling is important for convergent extension in the mouse notochord and neural plate, the results indicate that chato regulates body axis elongation in all embryonic tissues through a process independent of noncanonical Wnt signaling.
Keywords: signal transduction; controlled study; carrier protein; unclassified drug; gene mutation; genetics; missense mutation; mutation; nonhuman; mouse; animal; metabolism; animals; mice; allele; animal tissue; cell structure; gene expression; embryo; animal experiment; alleles; cell motion; embryo development; morphogenesis; physiology; animal embryo; regulatory mechanism; gene interaction; carrier proteins; cell movement; cellular distribution; embryo, mammalian; cell count; protein structure, tertiary; genetic disorder; structure analysis; wnt proteins; zinc finger protein; wnt protein; body patterning; protein tertiary structure; developmental stage; endoderm; danio rerio; gene activity; zinc fingers; neuroectoderm; notochord; embryo axis; mouse development; axis elongation; convergent extension; definitive endoderm; protein chato; chato protein, mouse; body axis elongation defect; body density; cell rearrangement; gestation period
Journal Title: Development
Volume: 135
Issue: 18
ISSN: 0950-1991
Publisher: Company of Biologists  
Date Published: 2008-09-01
Start Page: 3053
End Page: 3062
Language: English
DOI: 10.1242/dev.022897
PUBMED: 18701545
PROVIDER: scopus
PMCID: PMC2583081
DOI/URL:
Notes: --- - "Cited By (since 1996): 12" - "Export Date: 17 November 2011" - "CODEN: DEVPE" - "Source: Scopus"
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  1. Kathryn Anderson
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