Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo Journal Article
| Authors: | Garcia-Garcia, M. J.; Shibata, M.; Anderson, K. V. |
| Article Title: | Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo |
| Abstract: | In Xenopus and zebrafish embryos, elongation of the anterior-posterior body axis depends on convergent extension, a process that involves polarized cell movements and is regulated by non-canonical Wnt signaling. The mechanisms that control axis elongation of the mouse embryo are much less well understood. Here, we characterize the ENU-induced mouse mutation chato, which causes arrest at midgestation and defects characteristic of convergent extension mutants, including a shortened body axis, mediolaterally extended somites and an open neural tube. The chato mutation disrupts Zfp568, a Krüppel-associated box (KRAB) domain zincfinger protein. Morphometric analysis revealed that the definitive endoderm of mouse wild-type embryos undergoes cell rearrangements that lead to convergent extension during early somite stages, and that these cell rearrangements fail in chato embryos. Although non-canonical Wnt signaling is important for convergent extension in the mouse notochord and neural plate, the results indicate that chato regulates body axis elongation in all embryonic tissues through a process independent of noncanonical Wnt signaling. |
| Keywords: | signal transduction; controlled study; carrier protein; unclassified drug; gene mutation; genetics; missense mutation; mutation; nonhuman; mouse; animal; metabolism; animals; mice; allele; animal tissue; cell structure; gene expression; embryo; animal experiment; alleles; cell motion; embryo development; morphogenesis; physiology; animal embryo; regulatory mechanism; gene interaction; carrier proteins; cell movement; cellular distribution; embryo, mammalian; cell count; protein structure, tertiary; genetic disorder; structure analysis; wnt proteins; zinc finger protein; wnt protein; body patterning; protein tertiary structure; developmental stage; endoderm; danio rerio; gene activity; zinc fingers; neuroectoderm; notochord; embryo axis; mouse development; axis elongation; convergent extension; definitive endoderm; protein chato; chato protein, mouse; body axis elongation defect; body density; cell rearrangement; gestation period |
| Journal Title: | Development |
| Volume: | 135 |
| Issue: | 18 |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Date Published: | 2008-09-01 |
| Start Page: | 3053 |
| End Page: | 3062 |
| Language: | English |
| DOI: | 10.1242/dev.022897 |
| PUBMED: | 18701545 |
| PROVIDER: | scopus |
| PMCID: | PMC2583081 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 12" - "Export Date: 17 November 2011" - "CODEN: DEVPE" - "Source: Scopus" |
