Isolation of specialized plant cells by fluorescence-activated cell sorting Journal Article
| Authors: | Guedes, J. G.; Guimarães, A. L.; Carqueijeiro, I.; Gardner, R.; Bispo, C.; Sottomayor, M. |
| Article Title: | Isolation of specialized plant cells by fluorescence-activated cell sorting |
| Abstract: | Plant organs are built of different cell types, characterized by specific transcription programs and metabolic profiles. The possibility of isolation of such cell types to perform differential transcriptomic, proteomic and metabolomic analyses is highly important to understand many aspects of plant physiology, namely, the structure and regulation of economically valuable specialized metabolic pathways. Here, we describe the isolation of idioblast leaf protoplasts of the medicinal plant Catharanthus roseus by fluorescence-activated cell sorting, taking advantage of the differential autofluorescence properties of those specialized cells. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | flow cytometry; metabolism; proteomics; gene expression regulation; plant leaf; autofluorescence; plant leaves; gene expression regulation, plant; plant proteins; facs; plant cell; catharanthus roseus; alkaloid metabolism; idioblasts; mesophyll; plant cells; protoplasts; plant protein; catharanthus |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2469 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2022-01-01 |
| Start Page: | 193 |
| End Page: | 200 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-2185-1_16 |
| PUBMED: | 35508840 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Chapter 16 in "Plant Secondary Metabolism Engineering: Methods and Protocols. 2nd ed." (ISBN: 978-1-0716-2184-4) -- Export Date: 1 June 2022 -- Source: Scopus |
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