Biochemical characterization of a P43,12 complex: Comparison with human and murine class I molecules Journal Article
| Authors: | Bushkin, Y.; Chorney, M. J.; Diamante, E.; Lane, C.; Fu, S. M.; Wang, C. Y. |
| Article Title: | Biochemical characterization of a P43,12 complex: Comparison with human and murine class I molecules |
| Abstract: | A monoclonal antibody designated as C21 reacting with a p43,12 complex was developed against human thymocytes. It stained predominantly the early hematopoietic cells of the lymphoid lineage and also thymocytes, peripheral B-cells and activated T- and B-cells similarly to OKT10. The heavy chain of this antigen was a glycoprotein of M, 43,000 (p43). Sequential immunoprecipitation with C21 and OKT10 antibodies indicated that they both reacted with an identical heavy-chain molecule. This observation was further documented by two-dimensional analysis. Monoclonal antibody C21 was used to probe a p43,12 complex further. Structural polymorphism of the p43 heavy chain isolated from T- and B-cells of different individuals was not detected by chymotryptic peptide mappIng, although molecules from these cell types possessed a different charge on two-dimensional gels. An unusual observation was made regarding this complex on MOLT4 cells. The light chain co-precipitated from these cells was 12,000 daltons and had a pI distinct from that of β2-microglobulin but similar to the pI of the βt molecule. Comparison between chymotryptic peptide maps of the p43 heavy chain and those of the human and murine class I molecules such as HLA, T6, H-2K, Qa-2 and TL revealed no apparent homology. We have shown, however, that the peptide backbone of p43, as studied by both tunicamycin treatment of cells and endoglycosidase F digestion of immunoprecipitates, was identical in size to that of murine Qa-1. These results suggest that the p43 antigen may be homologous to murine Qa-1 or another class I antigen encoded in the murine TL:Qa region. © 1985. |
| Keywords: | t-lymphocytes; mouse; animals; mice; heredity; cell line; mice, inbred strains; lymphatic system; antibodies, monoclonal; amino acid sequence; antigens; lymphoma; hematopoietic system; major histocompatibility complex; biochemistry; carbohydrates; electrophoresis, polyacrylamide gel; major histocompatibility antigen class 1; hla system; humans; human; priority journal; leukemia, experimental; h2 system; immunoelectrophoresis, two-dimensional |
| Journal Title: | Molecular Immunology |
| Volume: | 22 |
| Issue: | 6 |
| ISSN: | 0161-5890 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 1985-06-01 |
| Start Page: | 695 |
| End Page: | 703 |
| Language: | English |
| DOI: | 10.1016/0161-5890(85)90100-2 |
| PROVIDER: | scopus |
| PUBMED: | 3875019 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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