Identification with the monoclonal antibody 26.163 of a determinant shared by the ß chains of the gene products of the HLA-DR, DQ, and DP loci Journal Article
| Authors: | Igarashi, M.; Gebhard, D. H.; Chen, Y. X.; Safai, B.; Evans, R. L.; Ferrone, S. |
| Article Title: | Identification with the monoclonal antibody 26.163 of a determinant shared by the ß chains of the gene products of the HLA-DR, DQ, and DP loci |
| Abstract: | Immunochemical studies (sequential immunoprecipitation followed by SDS-PAGE and two-dimensional gel electrophoresis) have shown that the monoclonal antibody (MoAb) 26.163 reacts with HLA-DR, DQ, and DP antigens. Testing with isolated α and ß chains of HLA class II antigens and immunoblot analysis also demonstrated that the determinant defined by the MoAb 26.163 is localized on ß chains and does not require their association with α chains for its expression. The MoAb 26.163 appears to be the first example of a monoclonal antibody with specificity for the gene products of HLA-DR, DQ, and DP loci. © 1986. |
| Keywords: | human cell; heredity; gene product; monoclonal antibody; antibodies, monoclonal; hla dr antigen; hla-dr antigens; immunoprecipitation; immunoblotting; hla-d antigens; chromosome mapping; electrophoresis, polyacrylamide gel; epitopes; hla dp antigen; paper; hla dq antigen; precipitin tests; humans; human; priority journal; hla-dp antigens; collodion; hla-dq antigens; immunoglobulin alpha-chains; blood and hemopoietic system |
| Journal Title: | Human Immunology |
| Volume: | 16 |
| Issue: | 4 |
| ISSN: | 0198-8859 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1986-08-01 |
| Start Page: | 347 |
| End Page: | 355 |
| Language: | English |
| DOI: | 10.1016/0198-8859(86)90061-3 |
| PUBMED: | 2428781 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: The authors wish to acknowledge the excellent secretarial assistance of Mrs. Edwina L. Jones and Mrs. Vicky Temponi. |
