Isolation and purification of the human thymocyte antigens T6 and M241 Journal Article
| Authors: | Lerch, P. G.; van de Rijn, M.; Smart, J. E.; Knowles, R. W.; Terhorst, C. |
| Article Title: | Isolation and purification of the human thymocyte antigens T6 and M241 |
| Abstract: | T6 and M241 antigens are products of the Class I major histocompatibility complex. The T6 and M241 antigens can be detected on human cortical thymocytes and on dendritic cells in the skin by monoclonal antibodies. Here we report a method of purification of the T6 and M241 antigens. Amino acid sequence data of purified antigens indicate that the heavy chains are blocked at their N-termini, whereas the partial N-terminal amino acid sequence of the light chains is identical to that of the human β2-microglobulin. In order to obtain sequence data from the heavy chains a method is described for isolation of purified cyanogen bromide fragments by electrophoretic methods. © 1986. |
| Keywords: | child, preschool; unclassified drug; human cell; t-lymphocytes; in vitro study; monoclonal antibody; antibodies, monoclonal; amino acid sequence; immunoglobulin heavy chains; thymus; infant; beta 2 microglobulin; major histocompatibility complex; immunoglobulin light chains; centrifugation, density gradient; antigens, surface; electrophoresis, polyacrylamide gel; antigens, differentiation, t-lymphocyte; purification; immunosorbent techniques; humans; human; priority journal; article; cyanogen bromide; thymocyte antigen |
| Journal Title: | Molecular Immunology |
| Volume: | 23 |
| Issue: | 2 |
| ISSN: | 0161-5890 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 1986-02-01 |
| Start Page: | 131 |
| End Page: | 139 |
| Language: | English |
| DOI: | 10.1016/0161-5890(86)90034-9 |
| PUBMED: | 3084948 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
