Molecular characterization of the HLA-Cw*0409N allele Journal Article


Authors: Wang, Z. C.; Smith, A. G.; Yunis, E. J.; Selvakumar, A.; Ferrone, S.; McKinney, S.; Lee, J. H.; Fernandez Vina, M.; Hansen, J. A.
Article Title: Molecular characterization of the HLA-Cw*0409N allele
Abstract: Various molecular methods in conjunction with serologic assays are used for clinical human leukocyte antigen (HLA) typing. Although serologic reagents detect most HLA-A and -B allospecificities, serologic HLA-C typing is hampered by the lack of informative antisera for many of the known HLA-C gene products. HLA antigens not detected by serology, but detected by molecular methods, are often referred to as "blank" antigens. Their lack of reactivity with antibodies in serological assays often reflects the presence of null alleles. The present study has characterized an HLA-Cw*04 allele (Cw*0409N) detected by DNA typing but not by serology. In cultured B-lymphoid 13W09501 cells carrying this Cw*04 null allele, isoelectric focusing analysis could not detect any component with a pattern compatible with that of the product of the HLA-Cw*0401 allele, but detected components reacting with an anti-HLA-Cw4 and Cw6 monoclonal antibody. Sequencing analysis of the full length HLA-Cw4 cDNA amplified from the cell line 13W095-01 revealed a base deletion at codon 365 in exon 7, resulting in a reading frame shift that added 32 amino acids at the C-terminal of the HLA-Cw4 heavy chain. These results indicate that the HLA-Cw*0409N allele may produce a putative long HLA-Cw4 heavy chain that is not expressed on the cell surface. © American Society for Histocompatibility and Immunogenetics, 2002. Published by Elsevier Science Inc.
Keywords: human cell; sequence analysis; polymerase chain reaction; allele; gene amplification; carboxy terminal sequence; alleles; amino acid sequence; molecular sequence data; molecular typing; hla-c antigens; graft versus host reaction; dna fingerprinting; base sequence; dna sequence; codon; hla a antigen; hla b antigen; hla c antigen; hla typing; null allele; dna, complementary; pcr; cell surface; dna sequencing; humans; human; male; female; priority journal; article; cw*0409n; hla null allele; ief
Journal Title: Human Immunology
Volume: 63
Issue: 4
ISSN: 0198-8859
Publisher: Elsevier Inc.  
Date Published: 2002-04-01
Start Page: 295
End Page: 300
Language: English
DOI: 10.1016/s0198-8859(02)00376-2
PUBMED: 12039411
PROVIDER: scopus
DOI/URL:
Notes: Export Date: 14 November 2014 -- Source: Scopus
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