Phenotypic characterization of ‘non‐T, non‐B’ acute lymphoblastic leukemia by a new panel (BL) of monoclonal antibodies Journal Article
| Authors: | Al‐Katib, A.; Wang, C. Y.; Bardales, R.; Koziner, B. |
| Article Title: | Phenotypic characterization of ‘non‐T, non‐B’ acute lymphoblastic leukemia by a new panel (BL) of monoclonal antibodies |
| Abstract: | Peripheral blood and/or bone marrow leukemic cell suspensions from 49 patients with ‘non‐T, non‐B’ acute lymphoblastic leukemia (ALL) were analysed by flow cytometry using a new panel of four monoclonal antibodies. Anti‐BL1 and anti‐BL2 originating from NALM‐6 and B35M lymphoblastoid cell lines, respectively. These antibodies recognize B‐cell differentiation antigens: a heat stable non‐immunoprecipitable antigenic determinant, and a 68 000 daltons glycoprotein molecule, respectively. BL5 and BL6 were derived by immunization with the promyelocytic cell line HL‐60, recognizing antigens present on early hemato‐poietic cells: an 85 000 daltons MW glycoprotein (Pro‐Im1) and a heat stable antigen (Pro‐Im2), respectively. All ALL patients studied had L1 or L2 morphology by the FAB classification and a blast count exceeding 50 per cent. There were 25 males and 24 females. Median age was 8 years (range 1–67 years). Thirty‐nine cases were studied at initial presentation and 10 at relapse. Cells from 46/49 cases expressed BL2 and/or BL1, but were not reactive with BL5 or BL6. Three of 49 cases did not express BL1 or BL2. However, a small percentage of blasts from one case was positive for BL5 (13 per cent) and the other 2 cases were reactive with BL6 (20 per cent and 36 per cent, respectively). These were one adult and 2 pediatric patients that had other ALL markers and achieved a complete remission with appropriate ALL therapy. One of the BL6+ cases relapsed after 19 months with a change in phenotype to BL1+ BL2+ BL5‐ BL6‐. This analysis shows that the majority of ‘non‐T, non‐B’ ALL's do express B‐cell associated antigens (BL1/BL2) argumentative of their B‐cell origin. A small subgroup does not express such antigens and may arise from a more immature cell, since they expressed antigens on early hematopoietic stem cells. Copyright © 1985 John Wiley & Sons, Ltd |
| Keywords: | adolescent; adult; child; aged; child, preschool; human cell; flow cytometry; t-lymphocytes; phenotype; cell line; acute lymphoblastic leukemia; b-lymphocytes; monoclonal antibodies; monoclonal antibody; lymphocyte differentiation; antibodies, monoclonal; antigens, neoplasm; infant; leukemia cell; hla-dr antigens; hematopoietic stem cells; histocompatibility antigens class ii; cell separation; epitopes; middle age; leukemia, lymphocytic; neprilysin; human; male; female; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; lymphoblastic leukemia; blood and hemopoietic system; immunoglobulins, surface; non b non t lymphocyte |
| Journal Title: | Hematological Oncology |
| Volume: | 3 |
| Issue: | 4 |
| ISSN: | 0278-0232 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1985-10-01 |
| Start Page: | 271 |
| End Page: | 281 |
| Language: | English |
| DOI: | 10.1002/hon.2900030406 |
| PUBMED: | 2417929 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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