Cellular distribution of a B-cell specific surface antigen (gp54) detected by a monoclonal antibody (anti-BL4) Journal Article
| Authors: | Hashimi, L.; Wang, C. Y.; Al-Katib, A.; Koziner, B. |
| Article Title: | Cellular distribution of a B-cell specific surface antigen (gp54) detected by a monoclonal antibody (anti-BL4) |
| Abstract: | A monoclonal antibody (anti-BL4) recognizing a previously characterized M r 54, 000 glycoprotein (gp54) was developed by immunizing BALB/ c mice with cells from a precursor B-cell line (Josh-7). In normal individuals, this antigenic molecule was present on tonsillar B-cells (60-80%) and on a fraction of peripheral blood B-cells (5-25%). BL4 (gp54) expression was investigated in 186 patients with a variety of hematological malignancies using indirect immunofluorescence and flow cytometric analysis. Twenty-six of 37 cases of B-cell chronic lymphocytic leukemia (CLL) and 18 of 33 cases of B-cell non-Hodgkins lymphoma were BL4 positive. Surface expression of BL4 on reactive cases of CLL and non-Hodgkins lymphoma was brighter than those of Bl, B2, and B4. BL4 positive CLL cases expressed a higher proportion of mouse rosette forming cells and Leu-1 positive cells than the BL4 negative subgroup and were not associated with elevated serum immunoglobulin levels. Four of 7 BL4 negative CLL cases were associated with increased serum levels of immunoglobulin M. Lymphoblasts from 14 of 14 cases of non-T acute lymphoblastic leukemia and 3 of 3 pre-B lymphoid blast crisis of chronic myeloid leukemia were BL4 negative. Neoplastic cells from 2 of 3 cases of Waldenstrom's macroglobulinemia and 4 of 7 cases of hairy cell leukemia were BL4 reactive. None of 7 cases of multiple myeloma and plasma cell leukemia were BL4 positive. All 11 T acute lymphoblastic leukemia cases, 6 other T-cell malignancies, 5 cases of Hodgkin's disease, 51 cases of acute nonlymphocytic leukemia, and 9 cases of chronic myeloid leukemia in chronic phase thus far studied were BL4 negative. An in vitro induction experiment using phorbol ester on a case of B-CLL demonstrated disappearance of BL4 accompanied with further B-cell differentiation. Our study further substantiates the previous finding that gp54 is a differentiation antigen restricted to the B-cell lineage and expressed during the intermediate stage of B-cell ontogeny. © 1986, American Association for Cancer Research. All rights reserved. |
| Keywords: | leukemia; human cell; major clinical study; flow cytometry; mouse; animal; mice; cell differentiation; immunofluorescence; chronic myeloid leukemia; mice, inbred balb c; b lymphocyte; b-lymphocytes; monoclonal antibody; lymphatic system; antibodies, monoclonal; nonhodgkin lymphoma; diagnosis; lymphoma; chronic lymphatic leukemia; glycoproteins; glycoprotein; antigens, surface; tetradecanoylphorbol acetate; antigens, differentiation, b-lymphocyte; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; nonlymphatic leukemia; blood and hemopoietic system |
| Journal Title: | Cancer Research |
| Volume: | 46 |
| Issue: | 10 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1986-10-01 |
| Start Page: | 5431 |
| End Page: | 5437 |
| Language: | English |
| PUBMED: | 3093065 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: The authors are most grateful to Dr. Jun Minowada of the Veterans Administration Hospital, Hines,IL, for providing the leukemic cell lines; to Patricia Black for her kind assistance in data gathering; and to Mary Lilly for typing the manuscript. |
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