Detection of intracytoplasmic immunoglobulin by flow cytometry in B-cell malignancies Journal Article
| Authors: | Bardales, R. H.; Al-Katib, A. M.; Carrato, A.; Koziner, B. |
| Article Title: | Detection of intracytoplasmic immunoglobulin by flow cytometry in B-cell malignancies |
| Abstract: | The aim of this study was to compare the results of flow cytometric (FCM) determination of heavy and light chain cytoplasmic immunoglobulin (cIg) with those obtained by the peroxidase-antiperoxidase (PAP) method. Fifty-one patients, including five non-T-acute lymphoblastic leukemias, 16 B-chronic lymphocytic leukemias (CLL), 13 non-Hodgkin's lymphomas, seven hairy cell leukemias, four multiple myeloma/plasma cell leukemias, and six T-cell leukemia/lymphomas, as well as 12 normal controls, were studied. Saponin-permeabilized cell suspensions were indirectly stained with monoclonal antibodies and analyzed by flow cytometry. Acetone-fixed cytocentrifuge smears were stained for cIg by the PAP method. The results obtained indicate that: (a) detection of cIg by FCM is a feasible and useful technique to confirm the B-cell lineage of leukemias and lymphomas, particularly those characterized by low-density surface immunoglobulin, such as CLL; and (b) cIg detection by FCM and PAP staining are complementary methods to recognize with certainty the monoclonality of B-cell malignancies. |
| Keywords: | leukemia; human cell; major clinical study; methodology; immunoglobulin; nonhodgkin lymphoma; immunoglobulin light chain; hairy cell leukemia; human |
| Journal Title: | Journal of Histochemistry and Cytochemistry |
| Volume: | 37 |
| Issue: | 1 |
| ISSN: | 0022-1554 |
| Publisher: | Sage Publications |
| Date Published: | 1989-01-01 |
| Start Page: | 83 |
| End Page: | 89 |
| Language: | English |
| DOI: | 10.1177/37.1.2491755 |
| PUBMED: | 2491755 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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