Distribution of a new B-cell-associated surface antigen (BL7) detected by a monoclonal antibody in human leukemic disorders Journal Article
| Authors: | Al-Katib, A.; Wang, C. Y.; Koziner, B. |
| Article Title: | Distribution of a new B-cell-associated surface antigen (BL7) detected by a monoclonal antibody in human leukemic disorders |
| Abstract: | A murine monoclonal antibody (anti-BL7) was raised by immunization of BALB/c mice with a precursor B-cell line (Josh-7) which detects a heat-stable, nonimmunoprecipitable antigen. The expression of BL7 was investigated in peripheral blood and/or bone marrow leukemic cell suspensions stained by indirect immunofluorescence and analyzed by flow cytometry. Lympho-blasts from 43 of 43 cases of “null” acute lymphoblastic leukemia were BL7~. Five cases of T-acute lymphoblastic leukemia and 5 cases of terminal deoxynucleotidyl transferase-positive blastic chronic myelogenous leukemia were also BL7′′. All 63 cases of B-cell chronic lymphocytic leukemia were BL7+ Neoplastic cells in 22 of 28 cases of B-cell non-Hodgkin's lymphomas in leukemic phase were also BL7+. Expression of BL7 showed some correlation with Rappaport's histological classification. Four cases of multiple myeloma and plasma cell leukemia were BL7-. Twenty-three cases of acute nonlymphocytic leukemias were also analyzed. Of these, only the acute promyelocytic (M3,4 cases) and acute myelomonocytic (M4, one case) varieties expressed BL7 on a small proportion (approximately 15%) of the leukemic cells. All other subgroups were BL7′′. The reactivity of anti-BL7 was compared to other B-cell antibodies on selected samples and was shown to be different from B1, B2, and the BA antibodies. Anti-BL7 is a unique monoclonal antibody useful in the study of B-cell cancers. © 1985, American Association for Cancer Research. All rights reserved. |
| Keywords: | leukemia; human cell; flow cytometry; animal; mice; multiple myeloma; cell line; immunofluorescence; chronic myeloid leukemia; mice, inbred balb c; histology; acute lymphoblastic leukemia; b lymphocyte; monoclonal antibody; lymphatic system; antibodies, monoclonal; lymphoma; antigens, surface; acute nonlymphocytic leukemia; antigens, differentiation, b-lymphocyte; leukemia, lymphocytic; human; priority journal; plasma cell leukemia; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; blood and hemopoietic system |
| Journal Title: | Cancer Research |
| Volume: | 45 |
| Issue: | 7 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1985-07-01 |
| Start Page: | 3058 |
| End Page: | 3063 |
| Language: | English |
| PUBMED: | 3924396 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
Citation Impact
Related MSK Work