Analysis of the specificity of five murine anti-blood group A monoclonal antibodies, including one that identifies type 3 and type 4 A determinants Journal Article
| Authors: | Furukawa, K.; Clausen, H.; Hakomori, S. I.; Sakamoto, J.; Look, K.; Lundblad, A.; Mattes, M. J.; Lloyd, K. O. |
| Article Title: | Analysis of the specificity of five murine anti-blood group A monoclonal antibodies, including one that identifies type 3 and type 4 A determinants |
| Abstract: | The specificity of five mouse monoclonal anti-A blood group antibodies (Ab), four of which were produced by immunization with cultured human cancer cells and one with a synthetic antigen, has been determined by examining their reactivity with purified A glycolipids, erythrocyte glycolipids, oligosaccharides, ovarian cyst glycoproteins, and salivary glycoproteins. Two of the antibodies (HT29-36 and CB) reacted with all A variant structures tested and have a broad anti-A reactivity. Ab CLH6 did not agglutinate A erythrocytes and reacted preferentially with the type 1A structure. Ab S12 agglutinated all A1 erythrocytes and reacted best with simple, monofucosyl type 2 A structures, such as Aa-2, Ab-2, and A tetrasaccharide. Ab M2 has a novel, but complex, spectrum of reactivity. It reacts with type 3 and type 4 A chains and not with type 1 and type 2 A chains. It appears to recognize both an external A structure [formula omitted] (I) (found) in type 3 and type 4 chains) and also an internal structure (II) found in type 3 chains. Ab M2 agglutinates all A and AB erythrocytes but does not react with salivary glycoproteins. © 1985, American Chemical Society. All rights reserved. |
| Keywords: | neoplasms; animals; mice; ovary cancer; cell line; blood group a; abo blood-group system; in vitro study; monoclonal antibody; antibodies, monoclonal; murinae; antibody specificity; glycoproteins; erythrocytes; female genital system; glycolipids; endocrine system; epitopes; oligosaccharides; hemagglutination; humans; human; priority journal; blood and hemopoietic system |
| Journal Title: | Biochemistry |
| Volume: | 24 |
| Issue: | 26 |
| ISSN: | 0006-2960 |
| Publisher: | American Chemical Society |
| Date Published: | 1985-12-17 |
| Start Page: | 7820 |
| End Page: | 7826 |
| Language: | English |
| DOI: | 10.1021/bi00347a047 |
| PUBMED: | 2418872 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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