Analysis of the fine specificities of 11 mouse monoclonal antibodies reactive with type 2 blood group determinants Journal Article
| Authors: | Furukawa, K.; Welt, S.; Yin, B. W. T.; Feickert, H. J.; Takashi, T.; Ueda, R.; Lloyd, K. O. |
| Article Title: | Analysis of the fine specificities of 11 mouse monoclonal antibodies reactive with type 2 blood group determinants |
| Abstract: | The specificity of 11 mouse monoclonal antibodies reacting selectively with type 2 blood group structures was analyzed in detail by studying their reactivities with a panel of standard glycolipids, glycolipids from erythrocytes and blood group glycoproteins. The antibodies reacted with monofucosyl type 2 H, difucosyl type 2 structures (Ley) or both; none of the antibodies reacted with type 1 (H, Lea, or Leb) structures. Only a small proportion of the antibodies were completely specific for either type 2 H or Ley structures. None of the antibodies had identical patterns of reactivity and their specificities were individually distinct. Seven antibodies preferentially agglutinated O and A2 erythrocytes. Anti-Ley-specific antibodies, except mAb101, did not agglutinate erythrocytes or react with glycolipids from erythrocytes, indicating the absence of Ley structures in erythrocyte glycolipids. The ability of some antibodies to react with A erythrocytes was shown to be due to cross-reactivity of the antibodies with type 3 (repetitive) A structures. The study demonstrates that monoclonal anti-carbohydrate antibodies tend to react with a range of related, and even distantly related, structures in a pattern characteristic of each antibody and that very few antibodies have extremely restricted specificities. © 1990. |
| Keywords: | unclassified drug; human cell; nonhuman; methodology; mouse; animal; mice; biological model; abo blood-group system; monoclonal antibody; antibodies, monoclonal; molecular sequence data; histochemistry; antibody specificity; erythrocyte; glycoproteins; carbohydrate sequence; erythrocytes; lewis blood-group system; glycolipid; glycolipids; epitopes; leukocytes; hemagglutination; saliva; human; priority journal; article; staphylococcus phage 3a; support, u.s. gov't, p.h.s.; blood group antigen; carbohydrate antibody |
| Journal Title: | Molecular Immunology |
| Volume: | 27 |
| Issue: | 8 |
| ISSN: | 0161-5890 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 1990-08-01 |
| Start Page: | 723 |
| End Page: | 732 |
| Language: | English |
| DOI: | 10.1016/0161-5890(90)90081-a |
| PUBMED: | 1698257 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
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