Specificity analysis of blood group Lewis-y (Le(y)) antibodies generated against synthetic and natural Le(y) determinants Journal Article

   


Authors: Kitamura, K.; Stockert, E.; Garin-Chesa, P.; Welt, S.; Lloyd, K. O.; Armour, K. L.; Wallace, T. P.; Harris, W. J.; Carr, F. J.; Old, L. J.
Article Title: Specificity analysis of blood group Lewis-y (Le(y)) antibodies generated against synthetic and natural Le(y) determinants
Abstract: Le(y)-reactive monoclonal antibodies (mAbs) were generated in mice by immunization with synthetic Le(y) neoglycoproteins or with Le(y)-expressing cells. Serological analysis indicated that mAbs raised against synthetic Le(y) (i) reacted strongly with synthetic Le(y) but poorly with natural Le(y), (ii) cross-reacted with Le(y) or H-type 2 structures, and (iii) were IgG1, IgG2a, or IgG2b, mAbs raised against Le(y)-expressing cells (i) reacted with both synthetic Le(y) and natural Le(y), (ii) were of two types: cross- reactive with Le(x) or H-type 2 structures or specific for Le(y), and (iii) were IgM or IgG3. One of the mAbs raised against natural Le(y), mAb 3S193 (IgG3), showed high specificity for Le(y) in ELISA tests with synthetic Le(y) and Le(y) containing glycoproteins and glycolipids; it also reacted strongly in rosetting assays and cytotoxic tests with Le(y)-expressing cells. mAb 3S193 did not lyse O, A, AB, and B human erythrocytes in the presence of human complement. In flow cytometry, there was weak reactivity with granulocytes, a reactivity also observed with two previously described highly specific Le(y) mouse mAbs-BR55-2 (IgG3) and B3 (IgG1). A humanized version of mAb 3S193 has been constructed, and the specificity pattern and reactivity for Le(y) remain very similar to mouse mAb 3S193.
Keywords: immunohistochemistry; nonhuman; flow cytometry; animal cell; mouse; animal; mice; tumor markers, biological; animal experiment; colonic neoplasms; tumor cells, cultured; animalia; monoclonal antibody; antibodies, monoclonal; molecular sequence data; antibody specificity; immunoglobulin g1; cross reaction; immunoglobulin g2a; antigen binding; antigens, tumor-associated, carbohydrate; carbohydrate sequence; in vitro; complement dependent cytotoxicity; lewis blood-group system; immunization; immunity, cellular; glycosphingolipids; cytotoxicity test; antigen antibody reaction; blood group lewis system; immunoglobulin g2b; glycoconjugates; humanized antibody; antibody-dependent cell cytotoxicity; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; blood group antigen; chimeric proteins; anti-le(y) antibody; synthetic neoglycoprotein; blood group antibody; complement hemolysis test
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 91
Issue: 26
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1994-12-20
Start Page: 12957
End Page: 12961
Language: English
DOI: 10.1073/pnas.91.26.12957
PROVIDER: scopus
PMCID: PMC45559
PUBMED: 7809154
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028609075&doi=10.1073%2fpnas.91.26.12957&partnerID=40&md5=4533f8be1b90f07f9d3ae6ad2b3a72b7
Notes: Export Date: 14 January 2019 -- Article -- CODEN: PNASA C2 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Lloyd J Old
    593 Old
  2. Kenneth O Lloyd
    163 Lloyd
  3. Sydney Welt
    98 Welt
  4. Elisabeth Stockert
    87 Stockert
  5. Pilar Garin-Chesa
    73 Garin-Chesa
Related MSK Work