Some monoclonal antibody reagents (C219 and JSB-1) to P-glycoprotein contain antibodies to blood group A carbohydrate determinants: A problem of quality control for immunohistochemical analysis Journal Article

  

Authors:	Finstad, C. L.; Yin, B. W. T.; Gordon, C. M.; Federici, M. G.; Welt, S.; Lloyd, K. O.
Article Title:	Some monoclonal antibody reagents (C219 and JSB-1) to P-glycoprotein contain antibodies to blood group A carbohydrate determinants: A problem of quality control for immunohistochemical analysis
Abstract:	Monoclonal antibodies (MAb) C219 and JSB-1 have been used extensively in the analysis of P-glycoprotein expression in normal and malignant tissues. This study demonstrates that some commercial lots of these MAb, even those supplied as purified immunoglobulins, contain contaminating anti-A blood group antibodies. In both sources of reagent, the antibody was specific for a particular A structure, known as repetitive or Type 3 A. These observations may account for earlier studies showing polymorphic variation in P-glycoprotein expression in epithelial tissues and an apparent correlation with the A blood type of the donor. Such reactivity can be eliminated by absorption of anti-P-glycoprotein reagents with A erythrocytes. These data re-emphasize the importance of evaluating MAb samples for unsuspected contaminating antibodies.
Keywords:	protein; carcinoma; multidrug resistance; p-glycoprotein; expression; ovarian-cancer; localization; gangliosides; tumor-cells; human-tissues; cancer; multidrug resistance gene; blood group-a; human ovarian; different epitopes
Journal Title:	Journal of Histochemistry and Cytochemistry
Volume:	39
Issue:	12
ISSN:	0022-1554
Publisher:	Sage Publications
Date Published:	1991-12-01
Start Page:	1603
End Page:	1610
Language:	English
ACCESSION:	WOS:A1991GR41400001
DOI:	10.1177/39.12.1682363
PROVIDER:	wos
PUBMED:	1682363
Notes:	Source: Wos

https://synapse.mskcc.org/synapse/works/has_related_data_chk/158637