Human melanoma proteoglycan: Expression in hybrids controlled by intrinsic and extrinsic signals Journal Article
| Authors: | Rettig, W. J.; Real, F. X.; Spengler, B. A.; Biedler, J. L.; Old, L. J. |
| Article Title: | Human melanoma proteoglycan: Expression in hybrids controlled by intrinsic and extrinsic signals |
| Abstract: | Human malignant melanoma cells express specific chondroitin sulfate proteoglycans (mel-CSPG) on the surface, both in vivo and in vitro. Melanocytes in normal skin show no detectable mel-CSPG but can be induced to express the antigen when cultured in the presence of cholera toxin and the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. Most other cell types do not express mel-CSPG either in vivo or in vitro. A study was designed to examine regulatory signals controlling mel-CSPG expression. The gene encoding mel-CSPG was mapped to human chromosome 15, and this chromosome was introduced into rodent cells derived from distinct differentiation lineages. Three types of mel-CSPG-expressing hybrids were found: (i) hybrids derived from human melanomas; (ii) hybrids derived from human cells that do not express mel-CSPG; and (iii) hybrids derived from human cells expressing mel-CSPG that are antigen-negative but that are induced to express mel-CSPG when cultured on extracellular matrix instead of plastic surfaces. Thus, mel-CSPG expression can be controlled both through intrinsic signals, provided by the differentiation program of the rodent fusion partner, and through extrinsic signals, provided by specific cell-matrix interactions. |
| Keywords: | human cell; animal cell; animals; mice; heredity; melanoma; gene expression; melanocytes; neoplasm proteins; cell line; animalia; gene expression regulation; antibodies, monoclonal; neuroblastoma; rat; rats; rodentia; lymphocytes; glycoproteins; chromosome mapping; phorbol 13 acetate 12 myristate; tetradecanoylphorbol acetate; cricetinae; cricetulus; lectins, c-type; aggrecans; hybrid cell; hybrid cells; proteoglycans; extracellular matrix proteins; proteochondroitin sulfate; proteoglycan; chromosome 15; humans; human; priority journal; cholera toxin; chromosomes, human, 13-15 |
| Journal Title: | Science |
| Volume: | 231 |
| Issue: | 4743 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1986-03-14 |
| Start Page: | 1281 |
| End Page: | 1284 |
| Language: | English |
| DOI: | 10.1126/science.3633135 |
| PUBMED: | 3633135 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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