Stimulatory effect of interleukin-1 upon hepatic metabolism Journal Article
| Authors: | Roh, M. S.; Moldawer, L. L.; Ekman, L. G.; Dinarello, C. A.; Bistrian, B. R.; Jeevanandam, M.; Brennan, M. F. |
| Article Title: | Stimulatory effect of interleukin-1 upon hepatic metabolism |
| Abstract: | The liver plays an important role in the acute-phase response to sepsis and injury, and host survival often depends upon an adequate hepatic response. Many of the metabolic sequelae to sepsis and injury are mediated by interleukin-1. This study was undertaken to investigate the impact of interleukin-1 upon hepatic metabolism and whether this mediator acted directly upon the liver. Interleukin-1 (5 rabbit pyrogen dose units) was administered to male Fisher F344 rats (175 to 200 g), and hepatocytes were isolated at three time periods; 2 to 4, 6 to 10, and 12 to 14 hours following an intraperitoneal injection. Alanine transport, gluconeogenesis, nonsecretory protein synthesis, and oxygen consumption were measured simultaneously in freshly isolated hepatocytes. Interleukin-1 stimulated initial rates of alanine uptake over a four-minute period. Peak stimulation of gluconeogenesis occurred at six to ten hours (0.52 ± .14 v 0.08 ± .01 nmol alanine converted/106 cells/min, P < 0.05); nonsecretory protein synthesis was significantly stimulated at 12 to 14 hours (2.1 ± .7 v 0.7 ± 0.1 nmol valine converted/106 cells/min, P < 0.05). These enhanced metabolic processes were associated with an increased oxygen consumption, with peak oxygen utilization occurring at six to ten hours (69 ± 2 v 25 ± 7 nmol of oxygen consumed 106 cells/min, P < 0.05). In order to examine if interleukin-1 exerted its effect directly upon the liver, hepatocytes from normal rats were incubated in vitro with this mediator for two hours. Under these experimental conditions, interleukin-1 did not reproduce the stimulatory effect obtained following in vivo administration. These data show that interleukin-1 stimulates many aspects of hepatic metabolism in a time-dependent fashion but may not act directly upon hepatocytes in a cell suspension. © 1986. |
| Keywords: | unclassified drug; drug efficacy; nonhuman; animals; animal experiment; time factors; liver; drug mechanism; protein synthesis; rat; protein biosynthesis; sepsis; rats; radioisotope; alanine; zinc; oxygen consumption; biological transport; injury; rats, inbred f344; interleukin 1; interleukin-1; gluconeogenesis; topical drug administration; liver metabolism; male; priority journal; article; valine c 14; alanine c 14 |
| Journal Title: | Metabolism |
| Volume: | 35 |
| Issue: | 5 |
| ISSN: | 0026-0495 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1986-05-01 |
| Start Page: | 419 |
| End Page: | 424 |
| Language: | English |
| DOI: | 10.1016/0026-0495(86)90131-9 |
| PUBMED: | 3486338 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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