Tumor necrosis factor alpha stimulates gluconeogenesis from alanine in vivo Journal Article
| Authors: | Blumberg, D.; Hochwald, S.; Burt, M.; Donner, D.; Brennan, M. F. |
| Article Title: | Tumor necrosis factor alpha stimulates gluconeogenesis from alanine in vivo |
| Abstract: | An increase in gluconeogenesis contributes to the cachexia seen in severe injury, sepsis, and malignancy by converting amino acids from skeletal muscle to glucose. Since tumor necrosis factor α (TNFα) may mediate this cachexia, we examined the effect of this cytokine on gluconeogenesis. Twenty‐eight male Fischer rats were injected intraperitoneally with TNFα (250 μg/kg) or saline, and after 4 hours, isolated hepatocytes were obtained by in situ collagenase liver perfusion. Hepatocytes were incubated with alanine (10 mM), and rates of gluconeogenesis were determined. Plasma lactate, glucose, insulin, glucagon, cortisol, and amino acids were measured. TNFα administration resulted in a 50% increase in gluconeogenesis from alanine (P <0.05) and a three‐fold increase in plasma glucagon (P = 0.01). Total and glucogenic plasma amino acids decreased with TNFα injection (P < 0.05). In vivo TNFα causes an increase in hepatic gluconeogenesis associated with increased plasma glucagon. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | controlled study; nonhuman; nutritional status; animal cell; animals; cells, cultured; animal experiment; chronic disease; cytokine; liver; tumor necrosis factor-alpha; rat; insulin; amino acid; insulin blood level; rats; cachexia; hydrocortisone; alanine; rats, inbred f344; hormone blood level; lactic acid; metabolic parameters; gluconeogenesis; amino acid metabolism; glucagon; intraperitoneal drug administration; liver metabolism; cancer; male; priority journal; article; tnfα; tissue survival; recombinant tumor necrosis factor alpha; glucagon blood level |
| Journal Title: | Journal of Surgical Oncology |
| Volume: | 59 |
| Issue: | 4 |
| ISSN: | 0022-4790 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1995-08-01 |
| Start Page: | 220 |
| End Page: | 225 |
| Language: | English |
| DOI: | 10.1002/jso.2930590404 |
| PUBMED: | 7630167 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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