Enhancement of umbilical cord blood cell hematopoiesis by maitake beta-glucan is mediated by granulocyte colony-stimulating factor production Journal Article

   

Authors:	Lin, H.; Cheung, S. W. Y.; Nesin, M.; Cassileth, B. R.; Cunningham Rundles, S.
Article Title:	Enhancement of umbilical cord blood cell hematopoiesis by maitake beta-glucan is mediated by granulocyte colony-stimulating factor production
Abstract:	Maitake beta-glucan (MBG) is an extract from the fruit body of the Grifola frondosa mushroom that is being widely used to treat cancer in Asia. We have previously reported that MBG enhances mouse bone marrow cell (BMC) hematopoiesis in vitro and protects BMC from doxorubicin (DOX) toxicity. In the current study, we investigated the ability of MBG to enhance hematopoiesis and to reduce the toxic effects of DOX on fresh human umbilical cord blood (CB) cells. MBG treatment significantly enhanced the colony formation unit (CFU) response of granulocytes-macrophages (CFU-GM response) over the whole dose range of 12.5 to 100 μg/ml (P < 0.05). The addition of MBG to DOX-treated CB cells significantly protected granulocyte-macrophage colony formation from the toxicity of DOX, which otherwise produced strong hematopoietic repression. MBG also partially replaced recombinant human granulocyte colony-stimulating factor (rhG-CSF), as shown by a significant augmentation of the CFU-GM response in the absence of rhG-CSF. We found that MBG induces granulocyte colony-stimulating factor (G-CSF) production in CB CD33+ monocytes, as detected by intracellular cytokine flow cytometric assessment. In contrast, we found that adult peripheral blood monocytes did not produce a significant G-CSF response to MBG, whereas both adult and CB monocytes produced G-CSF in response to lipopolysaccharide. These studies provide the first evidence that MBG induces hematopoietic stem cell proliferation and differentiation of CFU-GM in umbilical CB cells and acts directly to induce G-CSF. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Keywords:	controlled study; unclassified drug; human cell; doxorubicin; flow cytometry; cell proliferation; metabolism; cell differentiation; drug effect; physiology; biosynthesis; chemistry; fetal blood; fetus blood; hematopoietic stem cells; umbilical cord blood; lipopolysaccharide; cytokine production; hematopoiesis; hematopoietic stem cell; monocyte; beta glucan; recombinant granulocyte colony stimulating factor; grifola frondosa; grifola; isolation and purification; granulocyte colony stimulating factor; granulocyte colony-stimulating factor; peripheral blood mononuclear cell; maitake beta glucan; colony forming unit gm; beta-glucans
Journal Title:	Clinical and Vaccine Immunology
Volume:	14
Issue:	1
ISSN:	1556-6811
Publisher:	American Society for Microbiology
Date Published:	2007-01-01
Start Page:	21
End Page:	27
Language:	English
DOI:	10.1128/cvi.00284-06
PUBMED:	17093103
PROVIDER:	scopus
PMCID:	PMC1797710
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-34247855188&partnerID=40&md5=ea97b92d597d3ed097269df4e7c3e75c
Notes:	--- - "Cited By (since 1996): 19" - "Export Date: 17 November 2011" - "Source: Scopus"

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