Maitake beta-glucan MD-fraction enhances bone marrow colony formation and reduces doxorubicin toxicity in vitro Journal Article
| Authors: | Lin, H.; She, Y. H.; Cassileth, B. R.; Sirotnak, F.; Rundles, S. C. |
| Article Title: | Maitake beta-glucan MD-fraction enhances bone marrow colony formation and reduces doxorubicin toxicity in vitro |
| Abstract: | Previous studies have indicated that MD-fraction (MDF), in which the active component is beta 1,6-glucan with beta 1,3-branches, has anti-tumor activity as an oral agent and acts as an immune adjuvant. Since some other beta glucans appear to promote mobilization of hematopoietic stem cells, the effects of a beta glucan extract from the Maitake mushroom "MD-fraction" on hematopoietic stem cells were examined in a colony forming assay. Here we report for the first time that MDF has a dose response effect on mouse bone marrow cells (BMC) hematopoiesis in vitro. Using the Colony Forming Unit (CFU) assay to detect formation of granulocyte-macrophage (CFU-GM) colonies, and the XTT cytotoxicitiy assay to measure BMC viability, the data showed that the addition of MDF significantly enhanced the development of CFU-GM in a dose range of 50-100 μg/ml (p<0.004). The mechanism of action included significant increase of nonadherent BMC viability, which was observed at MDF doses of 12.5-100 μg/ml (p<0.005). In the presence of Doxorubicin (DOX), MDF promoted BMC viability and protected CFU-GM from DOX induced toxicity. In addition, MDF treatment promoted the recovery of CFU-GM colony formation after BMC were pretreated with DOX. These studies provided the first evidence that MDF acts directly in a dose dependent manner on hematopoietic BMC and enhances BMC growth and differentiation into colony forming cells. © 2004 Elsevier B.V. All rights reserved. |
| Keywords: | controlled study; doxorubicin; dose response; nonhuman; animal cell; mouse; animals; mice; cell viability; cell survival; cells, cultured; cell growth; granulocyte macrophage colony stimulating factor; cell differentiation; antineoplastic activity; cytotoxicity; in vitro study; dose-response relationship, drug; stem cell; hematopoietic stem cells; hematopoiesis; drug toxicity; mushroom; bone marrow cell; beta glucan; adjuvants, immunologic; antibiotics, antineoplastic; grifola; colony-forming units assay; cell protection; fungus; beta-glucan; beta-glucans; colony forming unit; colony forming cell; tetrazolium salts; colony formation; female; priority journal; article; cfu-gm; maitake mushroom |
| Journal Title: | International Immunopharmacology |
| Volume: | 4 |
| Issue: | 1 |
| ISSN: | 1567-5769 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2004-01-01 |
| Start Page: | 91 |
| End Page: | 99 |
| Language: | English |
| DOI: | 10.1016/j.intimp.2003.10.012 |
| PROVIDER: | scopus |
| PUBMED: | 14975363 |
| DOI/URL: | |
| Notes: | Int. Immunopharmacol. -- Cited By (since 1996):57 -- Export Date: 16 June 2014 -- CODEN: IINMB -- Source: Scopus |
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