High levels of granulocyte and granulocyte-macrophage colony-stimulating factors in cord blood of normal full-term neonates Journal Article

  

Authors:	Laver, J.; Duncan, E.; Abboud, M.; Gasparetto, C.; Sahdev, I.; Warren, D.; Bussel, J.; Auld, P.; O'Reilly, R. J.; Moore, M. A.
Article Title:	High levels of granulocyte and granulocyte-macrophage colony-stimulating factors in cord blood of normal full-term neonates
Abstract:	Because several human hematopoietic growth factors have been identified and shown to be effective for treatment of congenital or iatrogenic neutropenias, and cord blood contains stimulatory activities for blood-forming cells, we postulated that identification of these factors and analysis of their regulatory role in normal neonates would provide a rationale for their use in treating neonatal infections associated with neutropenia. We studied the plasma levels of granulocyte and granulocyte-macrophage colony-stimulating factors (G-CSF and GM-CSF, respectively) and the frequency of granulomopoietic colonyforming cells (CFU-GM) in the umbilical cord blood of normal term neonates. Plasma growth factor levels were measured by a biologic assay. Circulating hematopoietic progenitors were assayed for colony formation with different recombinant growth factors used as exogenous growth stimulators. The cell cycle status of these progenitors was analyzed by the thymidine suicide technique. At birth the leukocyte count (mean ± SD) was 11.0±3.9×109L and the neutrophil count was 5.6±2.6×109/L. The incidence of CFU-GM was significantly higher in umbilical cord blood than in normal adult peripheral blood (p<0.005) with up to 40% of the cells in S phase (<10% in normal adults). Plasma levels of G-CSF and GM-CSF at birth were 40.8±2.8 U/ml and 19.9±5.2 U/ml, respectively (normal adult plasma levels 2.5±1.5 U/ml for G-CSF and undetectable for GM-CSF). These high levels of G-CSF and GM-CSF in umbilical cord blood of normal neonates might play a role in maintaining adequate neutrophil production. © 1990 The C.V. Mosby Company.
Keywords:	adult; human cell; cell division; granulocyte macrophage colony stimulating factor; fetal blood; infant, newborn; recombinant proteins; newborn; stem cells; umbilical cord blood; cell count; hematopoiesis; blood cell count; macrophages; growth factor; granulocyte colony stimulating factor; granulocyte colony-stimulating factor; colony-stimulating factors; normal human; tumor necrosis factor; interleukin-1; growth substances; granulocyte-macrophage colony-stimulating factor; granulocytes; normal value; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title:	Journal of Pediatrics
Volume:	116
Issue:	4
ISSN:	0022-3476
Publisher:	Elsevier Inc.
Date Published:	1990-04-01
Start Page:	627
End Page:	632
Language:	English
DOI:	10.1016/s0022-3476(05)81617-8
PUBMED:	1690796
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025374076&doi=10.1016%2fS0022-3476%2805%2981617-8&partnerID=40&md5=184c0047bcdb2f884d679a14f1105e4c
Notes:	Article -- Export Date: 27 January 2020 -- Source: Scopus

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